Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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Determination of Cobalt Oxalate Hydtrate Introduction The objective of this lab is for students to determine the percentage of cobalt oxalate hydrate using the gravimetric analysis. They will also do a redox titration to determine the percentage of oxalate in the compound. After the percentages are calculated‚ you will determine the percent water by the difference of Oxalate and Cobalt from %100. Procedure Determination of Cobalt 1. Weigh 0.3 g of solid cobalt oxalate hydrate (from lab 8)
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Educational Preparation Jennifer Hansel Grand Canyon University: NRS-430V May 28‚ 2013 Educational Preparation The debate concerning the minimum education required by a Registered Nurse (RN) continues to be a hot topic throughout the years. There are multiple methods to achieve an RN degree. Many RN’s today have a two-year Associates Degree in Nursing (ADN) issued by a Community College‚ or a two-year Diploma Degree issued by a hospital; however‚ there is a palpable push towards RN’s obtaining
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the Composition of Cobalt Oxalate Hydrate Experiment 12 Robbie Kinsey Partner: Debnil Chowdhury Chem. 1312-D TA’s: Russell Dondero & Sylvester Mosley February 9‚ 2000 Purpose The purpose of this lab was to determine the percent cobalt and oxalate by mass‚ and with that information‚ the empirical formula for cobalt oxalate hydrate‚ using the general formula Coa(C2O4)b.cH2O. Procedure The powdered cobalt oxalate hydrate was
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Immediate Preparation - Marriage as a State of life I. Gaudium et Spes (#48-50) defines marriage as: 48. The intimate partnership of married life and love has been established by the Creator and qualified by His laws‚ and is rooted in the jugal covenant of irrevocable personal consent. Hence by that human act whereby spouses mutually bestow and accept each other a relationship arises which by divine will and in the eyes of society too is a lasting one. For the good of the spouses and their off-springs
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Experiment 2 Title: preparation of a halogenoalkane Objective: To synthesis 2-cholo-2-methylpropane by using corresponding alcohol and HCl(aq). The reaction is via SN1 of tertiary alcohol. Techniques: i) Distillation of the reactant and the product ii) Purification by separating funnel Theory: In the experiment‚ SN1 reaction take place‚ HCl is added to initiate the reaction. The R-OH attack the H+ with the lone pair on O. Then the R-O+H2 bond breaks the release a water molecule and
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EXPERIMENT A PREPARATION OF ASPIRIN Abstract This report gives a detailed account of the experimental preparation of aspirin in a laboratory environment as well as some basic industrial background on the product. It also contains information about safety precautions put in place to ensure the safety of the team who carried out the experiment. All results obtained have been included as well as a detailed analysis of what they represent alongside any improvements to the method used. Introduction
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been observed by her Mentor and associate Mentor‚ to fail in her actions to show competence in her practice whilst caring for the women. The key areas identified by her Mentor are: failing to explain her actions to a women in her care who has more complex needs‚ failed to gain verbal consent and failed to maintain privacy or dignity. Her associate Mentor has been concerned on shift with Allison as she noted that Allison did not follow universal precautions such as appropriate hand-washing or wearing
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approx.15 ml of 1mol/L Na2CO3 solution.Shaked‚drained and discarded the lower layer leaving the ester in the separating funnel. Diagram : Observations : From our results‚the ester we made produced a light banana smell. In this experiment for preparation of an ester as the alcohol used was pentanol and the acid used was glacial acetic acid‚ added conc.sulfuric acid a dehydrating agent used to remove the moisture‚ safety was a priority in this experiment as this were all highly poisonous‚highly
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Complex Carbohydrates PERSONAL SUMARY: There is a very real difference between simple and complex carbohydrates. Let’s look at them. Simple carbohydrates‚ found in rice‚ pasta‚ white potatoes‚ and some dairy products‚ contain sugars that are broken down quickly by your body. This will provide your body with a quick burst of energy‚ but if the carbohydrates are not used quickly‚ the sugar will turn to fat. That’s why low-carb diets cut them out. But those diets often lump all carbs together
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