Department of biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
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Oscillators & Oscilloscope Morgan Dennis @02514008 Partners: Brittany Smith‚ Michelle Trang‚ James Anderson‚ Madison Shaw OBJECTIVE: We are going to study the features and operation of the oscilloscope. We will use the instrument to measure the frequency and amplitude for various sources. Ultimately‚ the oscilloscope will display the Lissajous figures. APPARATUS: The major apparatus used include; Oscilloscope‚ two audio oscillators‚ battery and connectors. THEORY: The oscilloscope
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Introduction Memory is defined as the faculty by which the mind stores and remembers information(Eysenck & keane‚ 2010). Atkinson and Shiffrin (1968) (Cowan‚ Rouder‚ & Stadler‚ 2000)‚ came up with a model of sensory memory which stated not everything we perceive we process. Craik and Tulving (1975) developed an opposing theory in which they proposed the levels of processing theory‚ stating its not the processing but the type of quality of the processing that is performed‚ that determines the retention
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Title Simple‚ no more than 20‚ 15 ample Cross cultural drinking experience in 1st year students. Abstract Summary of the research: 100-150 words Introduction‚ method (drop of the design)‚ Results (inferential statistic‚ Why is it significant)‚ Discussion. Compose at the end. Introduction About 800 words Introduces the area theorem or topic etc. Evidence (pull out the criticism) Rationale H1: Extroverts have a higher pain threshold than introverts H0: Extroverts do not have a higher
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INTERPRETATION OF RESULTS: This experiment circles around with the Newton’s second condition of equilibrium in rotational motion. It describes by net torque acting on a body which is zero. The ability of the body to rotate in a certain direction is varied according on how much torque is applied. To prove that‚ a beam that is subjected to two forces is balanced by adjusting the perpendicular distances. When applied force is weight‚ modification in masses added is also done. Once equilibrium is achieved
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EXPERIMENT 5 SEPARATION OF A MIXTURE INTO ITS COMPONENTS BY FRACTIONAL CRYSTALLIZATION Introduction: Fractional crystallization is one of the experimental techniques used to separate or purify mixture. Fractional crystallization makes use of the differences of solubility to separate the components of a mixture. Goals: Applying fractional crystallization to separate a mixture of salicylic acid and copper sulfate pentahydrate into its components. Calculate the percent of salicylic
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Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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of three separated components Research Question: Is it possible to separate salt‚ sand and iron filings from each other in a mixture‚ to figure out the percentage of each component in the original mixture? Background Research: In this Investigation‚ I will be separating sand‚ salt‚ iron fillings in a mixture and finding their percentages. Iron is a magnetic solid which is incapable of being dissolved in water. Salt is a non-magnetic solid which is capable of being dissolved in water. Sand is
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