Chemistry II APPROVED: ___________ PREPARATION OF AZO DYES ABSTRACT In this experiment‚ the azo dyes p-nitrobenzene azoresorcinol and methyl orange were prepared by the azo coupling reaction. The p-nitrobenzene azoresorcinol dye was prepared from p-nitroaniline and resorcinol. The diazonium salt formed was from the reaction of the cold solution of dissolved p-nitroaniline in hydrochloric acid and water with the solution of sodium nitrite. Azo coupling was then followed by letting the
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Names:____________ Date Performed:_____ Experiment No:______ Score:_____________ AZO DYES PREPARATION INTRODUCTION AND OBJECTIVES The preparation of azo dyes is a standard reaction in an Organic Chemistry lab. Its adaptation to a parallel synthesis format is straightforward. Since the dyes vary widely in color‚ the diversity of this parallel synthesis becomes obvious. It includes techniques on diazotization and electrophilic substitiution. The experient belo is made to allow studens to
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The purpose of this lab was to synthesize indigo and an azo dye and to determine how effective each dye was to dye different types of fabrics. 0.168g of 2-nitrobenzaldehyde was dissolved into acetone along with 25 drops of distilled water and NaOH to synthesize indigo. The solution was filtered‚ and the solid indigo was placed into the filter flask to reduce indigo to leucoindigo with 10mL of 3M NaOH. The flask was heated along with sodium dithionite and distilled water added to the flask. Afiber
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cell membranes are discovered in this exercise. Background The following information is key for the interpretation of the results of this experiment 1. Neutral red is a vital stain. It stains cells without quickly killing them. Many biological stains kill living cells. 2. Neutral red goes through an obvious color change‚ from red at about pH 6.8 to yellow at pH 8.0. 3. A 1% solution of sodium bicarbonate has a pH of about 8.5. 4. Yeast cells have an internal pH that is slightly acidic (about
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The kermes dye was considered to be native to the old world‚ “Used by the Aztecs for dyeing and painting” (Douma‚ 2008)‚ and the Incas‚ who loved their reds. Throughout this time‚ various native american groups throughout Mesoamerica and South America used as dyestuff. It wasn’t until the 16th Century‚ that the Spanish conquest pushed the kermes dye into the hands of the Europeans. In fact‚ “When Spanish explorers discovered [the dye] and Conquistadors invaded Central and South America in the late
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Biology Lab report #1 The uptake of neutral red dye in a yeast cell using different solutions Abstract Every cell transports materials in and out throught something called a membrane. There are many different methods of transport in the cell Saccharomyces cerevisiae (Serrano‚ 1977) We want to know does adding higher concentrations of azide more effectively block dye transport? We tested the transport of dye in yeast cells with a metabolic inhibitor. When we did this we showed no difference
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The release of dyes through contaminated water into the ecosystem from various industries like textile‚ leather‚ cosmetic‚ paper‚ pharmaceutical and food industries is not only a source of aesthetic pollution but also can cause human health disorders and adversely affects the aquatic life causing severe environmental problems worldwide. In view of increasing concern from perspective of environmental safety and health‚ physico-chemical and biological techniques are to be constantly explored for decolourization
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Determining the concentration of red dye #40 in an unknown solution Introduction: White light is composed of many different wavelengths of light combined together. A spectrophotometer is an instrument that shines a single wavelength of light of a known intensity into a solution and then measures the intensity of the light exiting the solution. If a solution contains
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AZO-STANDARD Azo-standard is a drug which is used for the treatment of urinary tract infections (UTI)‚ Surgeries and injuries. This drug is available in tablet form 100mg and 200mg doses respectively. It contains chemical name as phenazopyridine hydrochloride. It has lot of positive results in market as well thousands of positive reviews online. This medicine is recommended by physician as no 1 drug for the treatment of urinary tract infections. There are many other drugs like it such as Baridium
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Determining the Concentrations of Red Dye in Sodas Abstract The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer‚ we measured how much light is absorbed by various known concentrations of red dye. After collecting this data‚ a standard curve was made that correlated the concentration of red dye #40 to its absorbance rate. Our
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