Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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BUFFERS By: Luis P. Bazan‚ RPh.‚ Ph.D. A buffer solution is a solution of: 1. A weak acid or a weak base and 2. The salt of the weak acid or weak base Both must be present! A buffer solution has the ability to resist changes in pH upon the addition of small amounts of either acid or base. Consider an equal molar mixture of CH3COOH and CH3COONa CH3COOH (aq) H+ (aq) + CH3COO- (aq) Adding more acid creates a shift left IF enough acetate ions are present 16.3 Which of the
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the temperature change. Method: 1. Set equipment diagram 2. Measure 30cm^3 water 3. Pour into polystyrene cup 4. Measure temperature 5. Weigh 1g Ammonium Nitrate 6. Put in cup 7. Hold and swirl beaker 8. Record min. temp. 9. Dispose solution separate beaker 10. Rinse cup 11. Repeat for 2g‚ 3g‚ 4g‚ 5g Equipment: Balance Ammonium Nitrate powder Distilled water Spatula Glass beaker 30cm^3 Measuring cylinder Weighing boat Thermometer 0C Polystyrene cup and lid with hole Risk
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Throughout the week in the toxicology lab‚ I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following: 1- hTSH test (Human thyroid stimulating Hormone): - Add calibration and +/- controls‚ before starting the punching process. - Add hTSH assay buffer + stock solution. - Put the plate on a fast quick shaking for 10mins‚ and afterward 4 hours moderate shaking at room T° -Add washing solution. -Add enhancement solution‚ and read the result. *The hTSH
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Solution A homogeneous mixture of two or more substances with each substance retaining its own chemical identity. Solute – substance being dissolved. Solvent – liquid water. General Properties of a Solution 1. Contains 2 or more components. 2. Has variable composition. 3. Properties change as the ratio of solute to solvent is changed. 4. Dissolved solutes are present as individual particles. 5. Solutes remain uniformly distributed and will not settle out with time. 6. Solute
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About Emirates On 25th October 1985‚ Emirates flew its first routes out of Dubai with just two aircraft—a leased Boeing 737 and Airbus 300 B4. Then as now‚ our goal was quality‚ not quantity‚ and in the years since taking those first small steps onto the regional travel scene‚ Emirates has evolved into a globally influential travel and tourism conglomerate known the world over for our commitment to the highest standards of quality in every aspect of our business. Though wholly owned by the Government
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Then this was used to find out how many mL of 0.5 NaOH solution is needed to react completely with all the copper (II) sulphate in solution. Then titrate ¼ of the estimated amount number of mL of NaOH needed to react with the sulphate solution. Then add 5 mL at a time NaOH until Cu(OH)2 precipitate does not form. Record the correct number off mL which forms to NaOH solution. Fold the filter paper and stick inside the funnel‚ and pour the solution of copper (II) hydroxide through the filter. The purpose
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Proper Preparation Prevents Poor Performance History and Overviews of Project Management As a discipline‚ project management developed from several fields of application including civil construction‚ engineering‚ and heavy defense activity. Two forefathers of project management are Henry Gantt‚ called the father of planning and control techniques‚ who is famous for his use of the Gantt chart as a project management tool (alternatively Harmon gram first
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KASPERSKY LAB BUS 260A Marketing FALL 2012 Final Project Aghakarim Orujov‚ Alina Kurisheva‚ Natalya Amirova‚ Solongo Bayarma‚ Sergey Nikolenko Date of preparation: September 9‚ 2012 Date of submission: December 6‚ 2012 Executive summary Kaspersky Lab is an international group operating in almost two hundred countries and territories worldwide. The company’s headquarters are located in Moscow‚ Russia‚ from where it oversees global operations and business development. Kaspersky Lab offers
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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