light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with other known concentrations‚ and then experimenting by observing how the concentrations were passed through the spectrophotometer. The outcome resolved in the absorption levels being decreased‚
Premium Concentration Serum albumin Absorbance
of dilutions were performed and the concentrations were calculated to find ‘E‚’ the molar absorptivity‚ which was determined to be 18035 M-1 cm-1. Introduction The experimental behavior of the absorption spectroscopy lab is to be able to determine the molar absorptivity of a food dye; in this case‚ Red-40. The determination of the best wavelength to use is found by measuring the highest peak that had an absorbance between 1 and 1.5. The dye concentration will be accomplished by preparing a series
Premium
Pre-Lab 1 Assignment Quantitative Analysis of Biomolecules Biochemical analysis involves the characterization of biomolecules within a sample using appropriate laboratory techniques. There are two principal approaches: 1. Qualitative analysis – where a sample is analyzed to determine whether a biomolecule is present or absent. As an example‚ a blood sample might be analyzed for a specific antibody or a bacterial cell might be probed for a nucleic acid sequence. 2. Quantitative analysis
Premium Analytical chemistry Concentration Glucose tolerance test
Professor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated the number of gram-negative enteric organism in 1 ml of alfalfa water. Exact colony counts from the experiment were
Premium Growth medium Bacteria Agar plate
Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
Premium Bacteria Nutrition Food
experiment allowed the students to perform the plate count technique by serial dilution and two common methods‚ spread plate and pour plate to determine the colony forming unit (CFU) of yeasts A ten-fold dilution is used in this experiment‚ the sample is diluted until it reached the 10-9 dilution. Plating for spread plate started from 10-5‚ 10-6‚ 10-7 and 10-8 dilution while for pour plate‚ it started from 10-6‚ 10-7‚ 10-8 and 10-9 dilution. Having incubated inverted at room temperature (25oC) for 2 days
Free Agar plate Petri dish Yeast
What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
Premium Chromatography Analytical chemistry Gas chromatography
see the effects of pasteurization while emphasizing the process for serial dilutions. PROCEDURE See references (1) RESULTS As the dilution factor increased for both the raw milk (unpasteurized) and pasteurized milk samples‚ the number of colonies decreased. The number of cells/mL in the pasteurized milk sample is considerably less than the number of cells/mL in the raw milk sample. RAW (UNPASTEURIZED) SAMPLE Dilution Factor | Number of Colonies | Number of cells/mL | 10-3 | TMTC |
Premium Milk Pasteurization Dairy product
Student number: 1701974 Unit code: BHS004-1 Assessment number: 2/5 Word count: You were given five samples of patients’ urine and some Uristix® dipstick. Describe how you used the dipsticks to determine if urine sample contained amounts of protein and / or glucose. To test the given samples for glucose or protein‚ dipsticks were immersed into each of the urine samples for 60 seconds. The colour change was compared with the colour chart on the Uristix® bottle. The detection of glucose in urine
Premium Enzyme Protein Blood
growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution‚we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth
Premium Bacteria Bacterial growth Cell division