The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture of Escherichia coli BL21 (see prior preparation) • A sample of Yakult (approximately 5 ml) • Marker pens to label plates & bottles • Sterile plastic loops for streaking bacteria (up to 20 per pair) • Sterile plastic spreaders for spreading bacteria (4 per pair) • Sterile universals/bijoux bottles (8 per pair) for serial dilutions • 10 ml sterile phosphate
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Each group had been assigned one of the two strains of the yeast for plating. Once the sample was obtained‚ the S. cerevisiae stock solution went through a set of serial dilutions. First‚ the stock solution was placed in a vortex machine to make sure the contents were thoroughly mixed. This process of vortexing occurred before every transfer of solution. Next‚ 100ul of the stock solution was added to a vial two that contained
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Some food in this world are made using microorganism to produce a desire flavour‚ taste and texture of the food. For examples: yogurt‚ tapai‚ cheese‚ bread and others. Starter cultures is used in these food production. A starter culture is a microbiological culture which actually perform fermentation. These starters usually consist of a cultivation medium‚ such as grains‚ seeds‚ or nutrient liquids that have been well colonized by the microorganisms used for the fermentation. These starters are formed
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is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell density) vs. time. Materials and Procedure: The materials that were used
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or four minutes for the precipitates to form. Observe the pattern of precipitation. Record. Part II Ksp by dilution of hydroxide ions. 1. Repeat the procedure in Part I‚ but use a serial dilution of NaOH followed by 5 drops of calcium nitrate in each well. Data Analysis Part I 1. Determine the concentration of the calcium ion in each well after the serial dilution but before the sodium hydroxide has been added. 2. Determine the concentration of the calcium ion in each
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Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant‚ it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently‚ this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is indicative
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buffer and Serial dilutions; which you make by diluting the 1% stock solution of amylase into 5 different concentrations (0.5‚ 0.25‚ 0.3125‚ 0.063‚ & 0.0315). Taking the 5 serial dilution tubes‚ you add to each serial dilution tube; 2mL of pH7 buffer‚ 1 mL of 1% starch solution‚ mixing immediately‚ than transfer a drop of the mixed solution into separate wells on the spot plate‚ watching the time to see how long it takes for the color to disappear‚ Repeat this for all the Serial dilutions and record
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Title: Counting the Number of Yeast Cells in a Suspension using Haemocytometer Objective: To estimate the number of cells of yeast per mm3 in five different dilutions of yeast suspension. Introduction: Biologists often need to count the density of cells in a liquid. “Density of cells” means “the number of cells per unit volume of liquid”. For example‚ they might want to find out the density of red blood cells in blood plasma‚ the density of bacteria in milk‚ or the population of Paramecium sp
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Their preference for these sweeteners vary depending on their concentration. To understand the specific concentration of sucrose preferred by Drosophila‚ we exposed them to a serial dilution of sucrose. After obtaining the concentration of sucrose that is most preferred‚ we created a serial dilution of quinine with this concentration to examine whether Drosophila could detect different concentrations of quinine. Since aging in humans is accompanied with a gradual loss of physiological
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protein of influenza viruses to sialic acid. The process requires the saturation of the surface of the virus with Abs. Stable neutralization - with time‚ Ag-Ab complexes usually become more stable (several hours) and the process cannot be reversed by dilution. Neither the virions nor the Abs are permanently changed in stable neutralization‚ for the unchanged components can be recovered. The neutralized virus can be reactivated by proteolytic cleavage. Stable neutralization has a different mechanism to
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