The litmus paper and the pH probe determined the salt solutions’ pH levels. When the blue litmus paper was dipped into ammonium chloride‚ the paper was changed to red whereas the red litmus paper stayed the same indicating that the solution was acidic. PH levels shown through pH probe also ranged between 5.5 to 6.0. Yet when in the presence of sodium acetate blue litmus stayed blue whereas red litmus turned blue‚ indicating its basicity. pH probe also showed the solution’s pH varied between the
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determine the effect of enzyme concentration and substrate concentration on the reaction rate‚ we performed a serial dilution of enzymes to get test tubes with full‚ one-half‚ one-fourth‚ and one-eighth concentrations. We then used a spectrophotometer to get the absorbance reading over time‚ and we used these readings to determine the reaction rate. We did the same thing with a serial dilution for substrate concentration‚ and we found the reaction rate for these as well. We found that as enzyme
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in this well‚ 2‚ is now 0.050 M in Ca2 ion.) Use your empty pipet to remove the solution from well 2 and put 5 drops of this solution into well 3. Put the remaining solution back in well 2. Mix the solution in well 3 as before. Continue this serial dilution procedure‚ adding 5 drops of the previous solution to the 5 drops of water in each well down the row until
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Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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To make the standard curve was made by serial dilutions. Human control DNA with known concentration was used: 1. First was diluted 1 to 11 and the concentration was 1ug in 5ul 2. 1ul of previous dilution in 9ul H2O > 0.01ug DNA in 5ul 3. 1ul of previous dilution in 9ul H2O > 0.001ug DNA in 5ul 4. 1ul of previous dilution in 9ul H2O > 0.0001ug DNA in 5ul 5. 1ul of previous dilution in 9ul H2O > 0.00001ug DNA in 5ul To make my DNA sample I used dilutions: 1. 1 to 10 2. 1 to 100 The master
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Introduction Bacterial transformation is the permanent alteration of a bacterial cell genotype as a result of its uptake and incorporation of foreign DNA fragments from external medium (Anthony et al‚ 2008). In addition to chromosome‚ bacterial cells often contain extrachromosomal DNA called plasmids which are capable of autonomic replication and antibiotic resistance (Dale & Simon‚ 2010). Plasmids can transport foreign DNA into host or other bacterial cells hence they are known as vectors.
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DCP & CE Title The effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Aim To investigate the effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Research Question Do the different dilutions of yeast cell suspension affect the number of yeast cells per cm3 that counted using haemocytometer under
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Activity 1.1.5: ELISA Introduction Given Sue’s diagnosis‚ all of the patients from the past two days need to be called back in for immediate testing. School officials are concerned about a possible outbreak of bacterial meningitis on campus. In order to diagnose bacterial meningitis‚ it is necessary to obtain a sample of cerebral spinal fluid using a spinal tap. Since this procedure is extremely invasive and painful‚ only those patients doctors feel are at greatest risk for the disease will
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the flask until the meniscus was at the 100 mark. Then I placed the cap on the flask and put it upside down and right-side up‚ mixing it completely. I also had to complete the calculations for the next concentrations using =; I did this for serial dilutions of .0008 mol/‚ .0006 mol/‚ .0004 mol/‚ .0002 mol/‚ and .0001 mol/ concentrations. How many of current concentration necessary for the next desired
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