experiment began‚ or was it found based on a similarly known variable so the epsilon value could be known for Beer’s law. Additionally‚ if the epsilon was found from a stock hemoglobin sample what was it’s concentration? Were the dilutions parallel or serial? What dilutions or epsilon value was found?. The experiment can still be conducted effectively without such information‚ but it would have made things easier. Question 2 Outline the procedure you expect to use to determine the pH in your rat’s
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spectrophotometer‚ cuvettes‚ 5mL volumetric pipettes‚ droppers‚ 1mL volumetric pipettes‚ safety goggles‚ rubber gloves‚ and aprons. Procedure Label six test tubes (1 through 5 and B for blank). A series of 5 test solutions of Ferric Chloride are made by serial dilution. Dilute 1Ml of the stock Ferric Chloride to 10 mL with de-ionized water to make a 0.01M solution. Place 1mL of the 0.01M Ferric Chloride solution in the first test tube
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Sterile pipettes and droppers 1) (Phage Serial-Dilution) a) Label broth tubes with dilution factors (1:10‚ 1:100‚ 1:1000‚ 1:10‚000‚ 1:100‚000). b) Pipet 1.mL of stock to the first dilution. Vortex. c) Pipet 1.mL of the 1:10 dilution to the 1:100. Vortex. d) Continue the 10X dilution through to the final concentration. 2) (Phage Plating) e) Label each of the BHI plates according to the various dilutions and one plate “control” which will not include
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An experiment to determine percentage of cell viability by using serial dilutions and a haemocytometer Aim The aim of this experiment was to determine accuracy of the cell count and how valid the result of the experiment will be. Materials and Methods Refer to Scientific and Laboratory skills practical booklet. (Pg. 10-Pg. 12) Results Table one: Raw data (Viable cells) Tube Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Average Count A 34 31
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water and 500 mL of C2H3N into a 1 L graduated cylinder. Carefully mix the solution and store for 30 days at r.t.p. (Hayes p. 8). 2.2.4 Preparing Florfenicol Strengthening Solutions 2 - 20 mg/mL of reference standard florfenicol solutions should be mixed with ethanol and stored for 14 days at standard conditions of temperature and pressure (Hayes p. 7). 2.2.5 Preparing Standard Stock Solutions of Florfenicol A stock solution of 1.0 mg/mL florfenicol should be used to develop calibration standards (at
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Retrieved from: http://www.wiley.com/college/spata/samplechapters/ch04.pdf Gopalakrishnan Ilstrup. D. M. (1990).Statistical methods in microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC358156/pdf/cmr00048-0031.pdf Isolation of bacteria by dilution techniques Sharma. D. V.‚ el tol. (2013). Antibacterial and cytotoxic activity of bacillus methylotrophicus-scs2012 isolated from soil. Retrieved from: http://www.jmbfs.org/wp-content/uploads/2013/02/jmbfs_0247_devsharma.pdf Wallenius World Health
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solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination
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e. Independent: Serial dilutions‚ or % concentrations (unless you are doing Chemistry‚ wherein you could use molar concentrations) could be used to find the optimum‚ and a few samples above the optimum should be done to observe the plateau. f. Control: Just use the same concentration throughout your samples‚ and make note of it in your Design. 4) Enzyme concentration: g. Independent: Depending on the form of the enzyme‚ you could do serial dilutions of digestive enzymes‚
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What happens when this enzyme meets up with its substrate? * * * * * 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? * * * * 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. * *
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etc.) Remember to cite information and put responses in your own words to avoid plagiarism (and loss of credit on the assignment). 1. Refer to step 2 in the protocol for lab 6. Calculate the concentration of the sample in tubes 2-5 for the serial dilutions in the chart below. You may wish to refer to http://www.wellesley.edu/Biology/Concepts/Html/standardcurve.html Tube number Water (ml) Blue Solution (ml) Total Final volume (mL) Concentration (mg/ml) 1 0 5.0 2.5 1 mg/ml 2 2.5
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