"Protein characterization by gel filtration chromatography" Essays and Research Papers

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    Receptor Protein

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    Receptor Protein – Protein that binds to a specific single molecule‚ enabling the cell to respond to the signal molecule. i.e. – The muscles of a person exercising can not contract without receptor proteins and signal molecules that tell the muscles when to contract and when to relax. Second Messenger – Signal molecule produced in response to the binding of a chemical signal. Acts as a signal molecule in the cytoplasm. Signal Molecule – Carries information throughout the body and to other

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    Protein Shakes

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    Protein shakes 1) Verifiable facts: the 3 common forms of protein shakes are whey‚ soy and casein protein. Protein shakes are useful when: - You’re growing - You’re starting a program - You’re amping up your workouts - You’re recovering from an injury Unverified statements or value claim: This whole part is unverified: So how can you tell if you’re already getting enough protein? Do the math. Recreational athletes need 0.5-0.75 grams of protein daily for every per pound of body weight Competitive

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    of Muscle Proteins to Infer Evolutionary Relationships of Mammals‚ Fish and Birds Abstract This experiment used electrophoresis to examine the makeup of muscle proteins from two mammals (cow‚ Bos taurus; bear‚ Ursus americanus)‚ two aves (chicken‚ Gallus gallus; turkey‚ Meleagris gallopavo)‚ and two fish (King Salmon‚ Oncorhynchus tshawytscha;  Albacore Tuna‚ Scombridae unclassified). The hypothesis was that as species have diverged evolutionarily‚ the makeup of their muscle proteins has also

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    Protein Synthesis

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    Sarah Khan Bivins-5th April 20th‚ 2013 Protein Synthesis Every day‚ you take in an abundance of different biomolecules; one of them being proteins. Have you ever wondered how proteins are made? They don’t just grow from a tree or fall from the sky‚ they are made through a process called protein synthesis. Protein synthesis is broken up in two two steps: transcription and translation. Transcription starts inside the nucleus when the DNA is unzipped by helicase. Following that‚ the mRNA nucleotides

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    Chromatography is the separation of compounds to allow us to see the different colours of that compound‚ for instance we may use chromatography in a school lab to see the real colour compounds of plants and grass‚ by using chemicals such as acetone we it will allow the object to break compounds and thus we can see the true colour of the grass. In today’s society there are many real world applications which use the chromatography technique in their line of work‚ an example of one of these applications

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    Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P

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    Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page 7 to page 10) Results: (I) Spectrophotometric Determination of DNA A260 = 3.923

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    white. It is because the protein in egg white undergoes denaturation‚ the cross linkage(the hydrogen bonds‚ ionic bonds and disulphride bonds) which maintain the protein shape destructed‚ so protein lose its tertiary conformation. This denaturing process is very important‚ because before protein can be used in digestion they must be unfolded. Part A: denaturation of egg white Aim: To examine the factors on the effect of denaturation of egg white. Principle: As protein denaturation can be cause

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    Chem Lab Chromatography

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    Gabriel Alizaidy Liquid Chromatography September 14‚ 2009 Objective: To separate the components of unsweetened‚ grape flavored Kool-Aid. Procedure: Using two syringes‚ inject different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work

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    Chromatography Lab Report

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    Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.

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