The two techniques that were used to create a DNA profile in this experiment were PCR and gel electrophoresis. The PCR is used to amplify the several DNA samples and gel electrophoresis is performed to separate the DNA fragments according to their size. [6] In the first part of the experiment‚ PCR amplification of the DNA templates was performed and the products obtained were used to perform gel electrophoresis. The process of PCR allows for the amplification of the DNA samples and the components
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Report subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed to an
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1. The central purpose of this paper is to describe all of the methods of how PCR was developed and the results of the experiments involving extraction and amplification of DNA (Mullis et. al. 1986). 2. PCR has the ability to isolate specific DNA sequences with the use of primers. This is done by denaturing the DNA (at 95o C) so it is able to anneal to the primers that specify a fragment to be amplified (Mullis et. at. 1986). These primes anneal to a specific sequence of DNA in order to amplify
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corresponding anthocyanidins using paper chromatography‚ and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g‚ followed by blackberries at 1.19 x 10-6 g‚ finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins‚ since it contained the most streaks on the chromatography paper. The main conclusion that can be
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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5.05 Ink Chromatography Basic Lab Components Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography‚ especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood‚ ink‚ or drugs. However‚ the more common use is to
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Holden Caulfield Characterization Chart Remember to use specific examples and cite page numbers! |Observations Holden Makes About Himself |Observations Others Make About Holden |Observations You Make About Holden | | Page 16 |Page 21 |Page 115 |
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any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography. Hypothesis I think that there will be a
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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EXERCISE 3: DIFFERENTIAL PROTEIN PRECIPITATION Vanessa Andrea Estepa University of Mindanao‚ College of Science and Mathematics ABSTRACT: Denaturation is the disruption in the original conformation of the protein wherein the secondary‚ tertiary and quaternary structures are all affected. Denaturation is brought about by various kinds of physical and chemical means; this includes the addition of strong acids‚ heavy metal cations‚ alkaloidal reagents‚ salting out and addition of organic solvents.
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