Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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Changez professes himself a ‘lover’ of America to his anonymous listener at the start of the novel. To begin with‚ for Changez‚ the journey from Pakistan to Princeton (and then to Underwood Samson) was about “a dream come true. Princeton inspired in me the feeling that my life was a Rilm in which I was the star and everything was possible.” Later‚ Changez calls the dream his “American dream.” Changez’s life before 9/11 is a
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Shakespeare Essay The play Macbeth written by Shakespeare goes under the genre tragedy. The term ‘tragedy’ has come to be used to refer to any serious dramatic representation in which the main character goes through a series of misfortunes that eventually lead to his downfall. The development of the protagonist can be explained or referred to five stages that Aristotle created. The first stage is exposition where the character is introduced to the audience with the information that is necessary
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EXTRACTION AND ISOLATION OF PROTEINS Background of the experiment Pure proteins are not readily available for study or use. There are certain methods that should be done in order to obtain proteins from its sources. The objective of this experiment is to isolate casein from milk and albumin from egg and to identify and explain the principles behind the methods employed for protein extraction. Results and Discussion There are many possible sources where we could get proteins. It can be from plants
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How does Williams present Blanche in scene 1? From the beginning of A Streetcar Named Desire‚ Tennessee Williams‚ it is evident that Blanche will demonstrate a contrasting persona to that of the other female voices‚ ‘her appearance is incongruous to this setting. She is daintily dressed in a white suit.’ The introduction to Blanche through the stage directions makes it apparent to the reader that Blanche will not blend in with her new surroundings and will believe herself to be superior to that
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David | | | Traits | Evidence | How trait is revealed | Curious | Questions the validity of The Definition of Man “Clearly there must be a mistake somewhere. Surely having one very small toe extra...couldn’t be enough to make her ‘hateful in the sigh of God...’?” (14)Asks where Sophie lives “Where’s your home?” (7)Recalls Aunt Harriet’s incident “It was as though she had been wiped out of every memory but mine” (75)Runs to see the Fringes people “I dropped what I was doing‚
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Intact protein and base hydrozylate have different color reactions when subjected into various tests. The protein used is Casein which was isolated from milk. Color reactions ‚in these test‚ gives off the information about the composition of a protein. Results often varies from being positive to one and negative to another since one has its peptide bond intact while the other is broken. The different tests that are used in this experiment are : biuret test‚ Sakaguchi test‚ ninhydrin test‚ xanthoproteic
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* Training the class on how to make a tasty protein shake * Main interest is working out and staying active * Before I get any further I want to list the training objectives and they are the process and ingredients used to make a shake‚ the benefits and common mistakes. * Just get a general view of the audience‚ HOW MANY HAVE TRIED WHEY OR SOY BASED PROTEIN BEFORE? AND YOUR EXPECTATIONS ON THE TASTE? * What you need to make a protein shake? Why Banana (sweet when ripe and added potassium
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During protein digestion‚ a water molecule is added which breaks down the carbonyl-carbon-nitrogen sin the peptide bonds of proteins causing the liberation of amino acids. This process is referred to as proteolysis. Water molecules break down the carbonyl-carbon-nitrogen single bond (peptide bond) that holds single amino acid molecules together (Caroline Ritchie‚ 2013. This process is called hydrolysis and is catalysed by protease. The three main protease enzymes produced during digestion are pepsin
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1. Theory of Character According to Abrams the meaning of character are the persons presented in a dramatic or narrative work‚ who are interpreted by the reader as being edowed with moral and dispositional qualities that are expressed in what they say - the dialogue - and what they do - the action (198 1:2) Therefore characters are important element in the story. The stories always introduce their characters to give a description to the readers. Character is commonly used in two ways (Stanton
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