Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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Daniel Bergey Lab 2: Proteins and Starches Purpose The purpose of lab 2 and both tests with proteins and starches is to determine which substance contains either protein or starch. Hypothesis Proteins: I predict that any substance I test that derives from a living organism is will test positive proteins. Any substance that isn’t from a living organism more than likely will test negative for proteins. Starches: I predict that any substance that contains any level of glucose will test positive
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Starch‚ one variable may be observed. 2. Biuret reagent will turn your skin brownish-purple. Explain why this occurs. Biuret reagent will turn your skin a brownish-purple because Iodine detects protein in a substance by changing the color to a brown or purple shade‚ and since your skin contains protein it will also change color. 3. When greasy food is spilled on clothing‚ why is it difficult to clean with water alone? Water cannot remove grease water is polar and grease is non-polar. They are
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Introduction Flash-Column Chromatography is another useful technique used to separate and purify complex mixtures of compounds. In this experiment‚ TLC is used to determine the best solvent system for the column. Due to Flash-Column Chromatography1 being an inexpensive technique‚ many scientist use this process for drug and natural product purification. Method By using the information from Experiment 6B‚ the TLC solvent system used was 30% EtOAc: 70% Hex. Experiment 7 was broken into two parts‚
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________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
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CHAPTER III NAIPAUL’S CHARACTERS Human destiny looms large in Naipaul’s works and is reflected largely in the primitive vitality of his art. Naipaul’s characters represent a world not moved by love but dominated by greed‚ conflict and futility. As a satirical artist‚ he aims to provide a kind of hurtful laughter that may offer catharsis but not redemption. Naipaul observes‚ “My world is more confused than that of the other writers; I’ve had to fit in as part of the background” (Drozdiak 17). It
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extra hydrogen bonds on top of each other‚ making extra-tough microfibrils that cannot be digested by most organisms. Proteins accomplish many different tasks‚ including structural support‚ storage‚ transport‚ movement‚ and defense against foreign substances. One noteworthy type of protein‚ called an enzyme‚ acts as a catalyst‚ speeding up chemical reactions in the cell. All proteins are composed of the same set of 20 amino acids‚ and each amino acid differs from the other only by the "R group" attached
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purification of proteins What are proteins? Proteins are the main building blocks of life. They are essential for the body and have many different roles . Proteins are made from sequence of amino acids. Protein structure is determined by their sequence of amino acids‚ which are linked by peptide link. Proteins are made from about 50 to 2000 amino acid residues. Figure 1 Why purify proteins? They are many reasons why a biochemist might want to purify a protein. For example to
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Experiment 2 Quantification of Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A
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