"Protein characterization by gel filtration chromatography" Essays and Research Papers

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    Chromatography

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    Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc

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    Chromatography

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    Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart

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    Chromatography

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    Title:The Chromatography Process of Expo Markers Abstract:This experiment is to uncover whether or not the Expo Markers are homogeneous or heterogeneous mixtures. The experiment will conduct filter paper being slightly submerged in a container of water and inspected for pigmentation changes. In the experiment the pigments will rise up the filter paper once exposed to the water (solvent). After the experiment‚ it concludes that the mixture is a homogeneous mixture due to the chemical

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    Gel Manicures

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    Marissa Arias Senior Rhetoric Graf-8 April 14‚ 2014 Gel II Manicure Many women and even some men love to get their nails done‚ but hate how much time it takes to dry and on top of that they are extremely easy to ruin and chip. There is a new type of manicure trending know as Shellac‚ Gel nails‚ or No chip. According to my cosmetology instructor Samantha Rudman‚ this process takes about 35 to 40 minutes but dries in seconds. Many individuals would love to know this process even the men that

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    LISMA RUHILA BT ALIAS Group: AS201 5A Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER 2012 OBJECTIVE * To study measure

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    Protein SCI/241 Protein A protein are organic compounds that made up of amino acids and are the building blocks of the cells in the body. Every cell in the body requires proteins to grow and repair themselves so these proteins are necessary for a healthy body and survival. Having so much protein can hurt your body so when you consume so much can get you sick and the right size of protein can keep you from getting sick. They’re five types of protein in everything that we eat. The largest class

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    associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary phase.

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    Gel Electrophoresis

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    In gel electrophoresis‚ DNA fragments move through a porous matrix made of agarose‚ a gelatin-like substance purified from seaweed. The agarose is melted like Jell-O® and then poured into a plastic tray to harden into a slab called a gel. A plastic comb inserted at one end while the gel is hardening forms wells where DNA samples can be placed. The DNA is mixed with a loading buffer that contains glycerol—this makes it heavier than water‚ so it will sink to the bottom of the well. The gel is then

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    Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample

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    J Sol-Gel Sci Technol (2011) 59:73–94 DOI 10.1007/s10971-011-2465-0 ORIGINAL PAPER Sol–gel derived organic–inorganic hybrid materials: synthesis‚ characterizations and applications Sadanand Pandey • Shivani B. Mishra Received: 28 January 2011 / Accepted: 8 April 2011 / Published online: 22 April 2011 Ó Springer Science+Business Media‚ LLC 2011 Abstract Organic/inorganic hybrid materials prepared by the sol–gel approach have rapidly become a fascinating new field of research in materials

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