We successfully adapted a commercially available protein detection assay for the quantiWcation of PEI. All experiments described below were performed with linear 25-kDa PEI (Polysciences‚ Warrington‚ PA‚ USA) that we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA)
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Why should we care about proteins? • Proteins perform structural and functional roles in the body • Your body uses them‚ to build and repair tissue. It is an important building block of bones‚ muscles‚ cartilage‚ skin‚ and blood. • Proteins are a major key in making hormones like insulin • Albumin‚ which is a protein made by the liver acts like the bloods 24-hour service that attaches to and transports fatty acids‚ calcium‚ and other substances through the circulatory system to cells throughout the
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Protein Article Research John Doe SCI 241 May 17‚ 2012 Jane Doe Protein Article Research This class has been very informative during the past few weeks. We have learned how to count calories‚ read nutrition labels‚ use a tracker to monitor our daily intake‚ carbohydrates‚ and learned about proteins and their effects. According to the readings in the text and the article proteins are the building blocks in the body. I learned that protein is found in all tissues‚ cells and organs of the body
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Fish phylogenetic tree based on protein size Amanda Reed Lab partners: Lecia Redwine‚ Kyle Hatcher TA: Baneshwar Singh Biology 117‚ Section 25 Tuesday 10:30 AM Introduction Although tree diagrams have been used since the days of Charles Darwin‚ biologists have only recently adopted the tree model of evolution to read and interpret phylogenies. One of the reasons for this is the confusion that often arises from using a tree model to describe a phylogeny (Baum‚ 2008a). Many people interpret
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Bacterial Transformation Bacteria and plasmid to produce Red Fluorescent Proteins Alejandra Lopez
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Determination of Protein Content Using Kjedahl and Titration 2.0 Introduction Proteins are polymers. They are the source of dietary amino acids and are used for growth and maintenance of living systems. They are costlier sources of energy compared to carbohydrates and fats and hence the human body utilizes proteins mainly for biosynthesis rather than as an energy source‚ though the energy yield is 5 kcal/g of protein. Twenty different types of amino acids occur naturally in proteins. Proteins differ
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four food apple sauce‚ carrot juice‚ cracker juice‚ and turkey juice contain protein‚ starch‚ or sugar. Between these four foods two are positive for sugar which are apple sauce and carrot juice. Sugar is an organic molecule of carbohydrate and so is starch. The starch was positive in two of the foods which are cracker juice and carrot juice. Protein is its own organic molecule and only one food tested positive for protein and it is the turkey juice. Introduction The “Food Lab” was used to answer
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BIURET FOOD TEST FOR PROTEINS molecules consisting of one or more chains of amino acids.Proteins are complex molecules that react differently to many compounds but are also fragile and cannot withstand high temperatures or strong acids and bases without degrading. The Biuret Method‚ or biuret test‚ is used to detect the presence of peptide bonds. It has this name because it reacts positively to the biuret molecule’s peptide bonds. Correctly evaluating the number of peptide bonds is a step towards
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