EXPERIMENT 1: Proteins -polypeptides compose of > 50 amino acids Functions 1. Enzymes or subunits of enzymes-enhancing the rates of reactions 2. Structural or Mechanical roles 3. Immune response roles 4. Storage and transport of substances 5. Source of amino acids for organisms that cannot synthesize amino acids naturally ISOLATION- disruption of cell membranes to release cell contents; separation for other contaminants 1. CENTRIFUGATION 2. SALTING OUT-water
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PROTEIN Lesson 1 All material designated for higher level only is presented in italics |Syllabus content to be covered: | | | |Composition | |Basic structure
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Proteins are arguably the most important things that people know the least about. As OpenStax CNX puts it‚ “Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules”. Proteins are “Macromolecules that contain nitrogen as well as carbon‚ hydrogen‚ and oxygen”(Miller‚ Kenneth R.‚ and Joseph S. Levine 48). Macromolecules are exceedingly large molecules that can be made up of several lesser molecules called proteins.
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Food technology FOOD QUALITY: * FOOD HANDINLING & STORAGE: 1. Food poisoning: * Incorrectly ‚ handled or stored is potentially fatal * Food poisoning – occurs when becoming sick after eating food that is poisonous. * Symptoms: * Nausea‚ vomiting‚ stomach cramps‚ severe- double vision. Paralysis. * FOOD DOES NOT HAVE TO LOOK OR TASTE BAD TO BE CONTAMINATED. * Bacteria are single cell organisms‚ rapidly in right conditions. * Bacteria grow and multiply
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this experiment to supposedly determine albumin and casein’s molecular weights respectively. The system used in gel electrophoresis consists of 3 major parts: a stationary phase‚ a mobile phase) and an electrical power supply. In SDS-PAGE the polyacrylamide gel serves as the stationary phase‚ the buffer (Tris-HCl in this case) as the mobile phase and an electrical source/ cathode and anode terminal. The aim of SDS-PAGE is to separate peptide chains in a protein sample according to molecular weight
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and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins based on molecular weight under the influence of an applied electrical field and then used to prepare for the Western Blot (#1 Lehninger). The support medium used is a polyacrylamide gel and‚ it also used sodium
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methods such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity
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BIOLOGY 22 MODULE 1 – Chemical Basis of Life v2.0 * Levels of Organization – biological functions are ultimately based on the properties of atoms and molecules * Subatomic particles – neutrons‚ electrons‚ protons * Atoms * Compounds * Complexes of compounds * Organelles – bodies within cells that perform specific functions * Cell * Specific combination of organelles * Can metabolize and reproduce * Least elaborate living structure * Significance
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T1 lines (G1‚ G2‚ G3‚ G4 and G5) maintained in greenhouse were used for extraction of soluble protein. Lectin was extracted from leaves (2g) in 2 ml of TBS containing 0.25mM isopropylthio-ß-D-galactoside (IPTG; Merck) for overnight at 4 °C‚ the homogenate was centrifuged at 6700g for 20 min at 4° C. The supernatant collected was used for hemagglutination assay. The concentration of total soluble protein was determined by the Bradford method (1976).Further the quantity of recSRL expressed in transgenic
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Capparis spinosa L. belongs to the family Capparaceae is distributed throughout India and commonly known as Maratimokku in Tamil. In the present study attempts were made to assess the phytochemical‚ nutraceutical and chemical composition‚ pharmacological properties and safety profiles of C. spinosa. Powder microscopy revealed characteristics features of C. spinosa. Preliminary phytochemical screening of flower buds of C. spinosa L. revealed the presence of alkaloids‚ reducing sugars‚ carbohydrates
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