Brad is a 37 year old Caucasian male that entered the Emergency Department after experiencing a seizure in the middle of the night. The seizure was described by his wife as generalized‚ lasting approximately two minutes with tonic-clonic movements along with a loss of unconsciousness and function of his bladder. Brad reported that he has had headaches that have been unresponsive to over the counter medication over the last four month and his wife also notes that he has had an increased loss in energy
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allocated to my BABS1201 laboratory group. In the end‚ I was unable to find any primary literature sources that would help with the assignment but I did find an article that I thought would be appropriate. The journal article I chose is titled Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosa from the peer-reviewed journal‚ Microbiology. The over-arching field of study which the article falls under is Microbiology (there was a small clue in the title of the journal)
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Lab Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of
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Exercise 1: Cell Transport Mechanisms and Permeability Worksheet Student Name: Student ID# Student instructions: Follow the step-by-step instructions for this exercise found in your lab manual and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Rename this document to include your first and last name prior to submitting‚ e.g. Exercise1_JohnSmith.doc. Please make sure that your answers are typed in RED. Grading:
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only a small fractioncirculates the blood in a free state. Most testosterone circulates bound to two proteins‚ sex hormone binding protein (SHBG)‚ and albumin. Total testosterone levels are a combination or SHBG-bound‚ albumin-bound‚ and free testosterone. The bioavaible form (form that can bind to receptors) of testosterone includes that bound to albumin and the free fraction. On average albumin holds 54%‚ SHGB 44% and free 2% so it appears that 56% of testosterone is in the bioavaible state (2)
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translucent grease mark on a brown paper bag. The objective for the Dye test was to test for lipids. When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a cracker that
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EXPERIMENT: Biological Molecules Here are your goals for this lesson: • To conduct tests for glucose‚ starch‚ lipids and proteins • Use these test results to identify the biological molecules found in food Student Name: Brittany Gerdes Date Experiment done:12-20-12 Date Report Completed: 12-20-12 Name of Grader: Hypothesis: A prediction of what you think the results of the project will be. Write your hypothesis before you begin the experiment and‚ if the experiment has
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polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? The percent acrylamide refers to the size of the pores as percent acrylamide increases the size of the pores decreases. 2. Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel
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oxide precipitate. turbidimetric method protein in urine Quantitative Tests for 24-Hour Specimens.Trichloroacetic acid (TCA) test. The addition of TCA to a urine specimen precipitates the protein in a fine suspension that is quantified spectrophotometrically at 420 nm (nanometers) by comparison with a similarly treated standard. Heat and acetic acid method Heat test: Based on the principle of heat coagulation and precipitation of proteins. If any turbidity appears‚ add 2 drops of
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formation of benzoquinone‚ which is a brown substance. Benzoquinone molecules bond‚ forming melanin‚ which is what you see as the dark spots on bruised fruits and vegetables. Enzymes are sensitive to changes in H+ concentration. Being most enzymes are proteins‚ they have a large number of
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