Blood Disorder and Components Adrienne Lynch HCA 240 Group ID: AAGR0P54X7 Richard Jones 05/23/2010 ------------------------------------------------- In each body‚ human or animal there is a blood flow that circulates throughout the body that helps them to maintain life. It is a fluid that sustains the internal organs to continue to give nutrients for the body to continue to live. Blood is one of the essential parts of the body functions. We need blood to supply nutrient to other organs
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Transmittance and Recovery calculations 1. Enzyme denaturation can occur during salting out; what does this mean? Water-soluble proteins have concentric "shells" of semi-ordered water molecules arranged around them‚ in much the same way that dissolved salts have associated water molecules making them soluble. If you add too much salt‚ the waters in the protein solvation shells are stripped out to dissolve the salt‚ precipitating the protein out of solution. High salt on the outside relative to
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sufficient amounts to lead to lead to saturation of all enzyme-protein molecules per cell [6]. Other effects observed include structural changes in bacterial cell walls and intracellular and nuclear membranes as well as bacterial DNA and RNA denaturation‚ inhibiting replication [6‚110‚111]. Possibly these effects in bacterial RNA and DNA are related to (or in addition to) the observed effects on mitochondrial respiration and cytosolic protein that lead to bacterial cell death. The distinct activity of
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A protein standard curve was generated by using the protein standard (1 ampule) measurement at 595 nm. Standard curve was prepared as shown in Table 4. Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively. 3.10.2 Protein Determination
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Three types of lipids in foods: 1) Triglycerides Composed of: * Three fatty acid molecules * Fatty acids are long chains of carbon atoms surrounded by hydrogen atoms. * One glycerol molecule * Glycerol is a three-carbon alcohol that is the backbone of a triglyceride Stored in fat cells - Unsaturated fatty acids: - Do not stack well together - Liquid at room temp - Present in plants oils Hydrogenation: the addition of hydrogen atoms to unsaturated fatty acids
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attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? a. The glucose was able to diffuse because its molecular weight was less than 200‚ while the albumin was much larger than 200 and could not diffuse. My prediction was wrong as I originally thought that the MW of glucose was higher than 200. 4. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride‚ albumin‚ and urea. a. The molecular weight
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this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus will be connected
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References: Dr Cruzan’s Math (2012) The Most Important Solvent. [Online]. Available from http://www.drcruzan.com/Water.html [Accessed 3rd May 2014] Encyclopaedia Britannica (2014) Protein denaturation. [Online]. Available from http://www.britannica.com/EBchecked/topic/479680/protein/72545/Protein-denaturation [Accessed 4th May 2014] Endomeme (2014) Newton’s Law of cooling. [Online]. Available from http://www.endmemo.com/physics/coollaw.php [Accessed 4th May 2014] Engineering ToolBox (2014)‚
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Available at: http://academic.brooklyn.cuny.edu/biology/bio4fv/page/enz_act.htm [Accessed: 20 Jun 2013] BBC UK. n.d. Graph of enzyme activity against temperature. [image online] Available at: http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa/proteins/proteinsrev3.shtml [Accessed: 20 Jun 2013] Biotek. 2007. Kinetic reaction of various ß-galactosidase enzyme concentrations. [image online] Available at: http://www.biotek.com/resources/articles/kinetic-analysis-beta-galactosidase.html [Accessed:
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small membrane. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? We predicted that glucose and albumin would both diffuse very slowly through the 200 MWCO because they are large molecules. The results were; the glucose diffused through the 200 MWCO membrane at a rate of 0.0040 per minute. The albumin was not able to diffuse through the 200 MWCO because it is too large of a molecule to
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