We were able to determine this by the presence of simple sugar‚ protein and lipids which are all found in a typical meal at Buffalo Wild Wings. The way we could so easily determine this is through the standards we have established so we were able to compare our mystery meal substance to a constant. Even though we did
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The primary antibody recognizes a specific amino-acid sequence of a particular protein. In this case‚ the antibody was anti-cow albumin. Then‚ it was incubated at 37°C for 30 minutes. After incubated‚ it was rinsed with TBS +NP 40 1x for 4 times. Each time was 5 minutes. This step was to wash the unbound antibodies. Then‚ it was rinsed two times with TBS 1x
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were investigated to make a covalent binding between the iron oxide magnetic core and the immobilized enzyme. Immobilization using chitosan and glutaraldehyde show the best result. Finally the immobilization efficiency was tested by determination of protein concentration in a solution before and after mixing with the magnetic nanoparticles. Introduction In the last two decades‚ new terms with the prefix `nano’ have rushed into the scientific vocabulary; nanoparticle‚ nanostructure‚ nanotechnology
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prevents blood loss by: • Activating plasma proteins and platelets • Initiating clot formation when a vessel is broken • Blood prevents infection by: • Synthesizing and utilizing antibodies • Activating complement proteins • Activating WBCs to defend the body against foreign invaders 2. Specify the composition and functions of plasma. • Blood plasma contains over 100 solutes‚ including: • Proteins – albumin‚ globulins‚ clotting proteins‚ and others • Nonprotein nitrogenous substances
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bound of BSA-PR at pH 4 (61.543%) was highest compare to other pH. pH 6 has the percent bound of 3.569%‚ pH 8 has the percent bound of 2.330%‚ and pH 10 has the percent bound of 2.309%. pH 6‚ 8‚ and 10 do not have an optimal binding for bovine serum albumin and phenol red because they have very low percent bound (refer to table 1)‚ mostly is the free phenol red. Therefore it matches with the prediction. Moreover‚ there were 2 peaks presented when graphing. Based on the order of elution‚ the first peak
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Protein purification From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function‚ structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines
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chemical reactions that occur in perfect synchronization. Also‚ these reactions take place at an incredibly fast rate; however‚ without enzymes‚ these reactions could possibly take several years to complete. An enzyme is a macromolecule‚ generally a protein‚ that lowers the activation energy of a reaction without being changed by the reaction‚ and this causes the reaction to occur much faster than usual (Campbell et al.‚ 2014). The act of speeding of a chemical reaction is called catalysis‚ and molecules
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Kwashiorkor is a disease that is caused by malnutrition when there is not enough protein in the diet. Other names for Kwashiorkor are protein malnutrition‚ protein-calorie malnutrition‚ and malignant malnutrition (Kwashiorkor‚ 2011). Kwashiorkor is most common in areas where there is famine‚ limited food supply‚ and low levels of education. This disease can occur when people do not understand how to eat a proper diet. In very poor countries‚ this disease is more common. Often Kwashiorkor occurs during
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This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2) electrical separation‚ and (3) chemical denaturation. In the first step‚ the DNA sample is digested with three restriction enzymes that were picked as part of a triple digest plan. The result of which can be used to make a restriction map of the DNA of interest. Secondly‚ after the digestion of the
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Goodpasture’s syndrome (GS) is a rare disease‚ identified by Dr. Ernest Goodpasture in 1919 He described the syndrome during the influenza pandemic of 1919 when he reported on a patient who died from bleeding in the lungs and kidney failure. The incidence of Goodpasture is estimated to 1 case per million per year. This disease occurs more commonly in white people than in black people and is widespread in men than in women. Goodpasture syndrome is a pulmonary-renal syndrome‚ which is a group of acute
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