Carbohydrates‚ Proteins‚ lipids‚ and nucleic Acid Lab Exercise 6 Date: 9/17/12 Bio 102-11 Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound. Background Most organic compounds in living organisms are carbohydrates‚ proteins‚ lipids‚ and nucleic acids they are called macromolecules
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The role of protein in hormone enzyme functions Proteins are very important particles in our cells. Proteins are included in basically all bodily cell functions‚ including cellular regeneration and repair‚ tissue maintenance and regulation‚ hormone and enzyme production (James‚ C.S. Delores). They vary in structure as well as function. Talking about hormone and enzyme production‚ it is important to understand that amino acids are the basic components of hormones‚ which are critical chemical
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intellect‚ that are not afforded by a more complex and stimulating diet.” Proteins In this chapter‚ Ellen G. White talks about protein and how vital it is to our lives. First she talks about nuts and foods associated with nuts. How they are to be prepared with a certain “caution” and it should inexpensive. It emphasizes nature giving us plenty of food to digest and fulfill our needs of essential nutrients such as protein. Then she goes on tell us to be very careful in the dosage or proportion of
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Biochemistry: Biological Molecules Pg. 2 – 7 Carbohydrates Pg. 8 – 12 Proteins Pg. 13 – Lipids Carbohydrates. L.O. 1.1 and 1.2 Carbohydrates are sugars and they are the primary source of energy for our vital functions such as breathing and sleeping. There are two types of carbohydrates‚ simple and complicated. Monosaccharides‚ disaccharides and polysaccharides. L. O. 1.2 Carbohydrates are split into different classifications depending on how many molecules they have. Monosaccharide: one sugar
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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depending on the way the introns are removed‚ and if there are different amino acids produced during translation in the ribosome‚ then there will be unique proteins created‚ too. This allows there to be more types of proteins in an organism than types of genes in an organism’s genome. Repressor proteins can influence the regulation of protein synthesis by binding to operators (the segments of DNA that act as switches to either allow or prohibit RNA polymerase to create mRNA strands) in an operon
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Ricin is a toxic protein found in castor oil plant seeds. The toxicity of Ricin has been known for centuries and was even used by the KGB government to assassinate Georgi Markov‚ a famous journalist who openly criticized the Soviet Union’s communist regime. The toxicity of ricin is caused by its ability to deactivate ribosomes and halt protein synthesis. Therefore‚ Ricin is categorized as a ribosome inactivating protein (RIP). There are two types of RIPs: type I and type II. Type I RIPs are usually
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experiment‚ we will be testing three different proteins to see if they denature at the same temperature. A protein is a molecule containing amino acids created in ribosomes that have many functions‚ including being an enzyme and transporter‚ structuring‚ translating genetic information‚ and serving as a channel for charged molecules to pass through the cell membrane. As a molecule‚ a protein is shaped like a coil (Food Science Basics 2014). Denaturation is when a protein’s shape changes due to the
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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