The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction The Difficulty Limited and Extensive choices pose: Its Effect on motivation and Satisfaction Teagan. R. Donnelly 214282075 HPS121 Psychology B: Individual and Social Development The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction Abstract The conducted study is based on personal choice in limited and extensive choice experimental
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Purpose: To observe epithelial tissue by using a microscope to compare prepared slides. Introduction: Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as
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1. OBJECTIVES (a) To determine the equivalent circuit parameters of a 3-phase squirrel-cage induction motor from laboratory test data. (b) To determine the performance characteristics of an induction motor under load conditions. (c) To control the no-load speed of an induction motor. 2. INTRODUCTION 2.1 Three-phase squirrel-cage induction motors An induction motor is a transformer with a rotating secondary winding. It has two essential components: an outer stationary stator and an
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EXPERIMENT 1: TITLE: ISOMERISM AND KINETICS IN COORDINATION CHEMISTRY OBJECTIVE: 1. To prepare and study the kinetics of the interconversion of some of the isomers of a typical coordination compound. INTRODUCTION: Isomerism played a central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance
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Synthesis of Butyl Benzoate Using Phase Transfer Catalysis The objective of the experiment is to synthesize the butly benzoate by nucleophilic substitution and characterize it by IR spectroscopy. The percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask
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CHM 3411 – Problem Set 6 Due date: Wednesday‚ March 23rd. Do all of the following problems. Show your work. 1) Consider the cyclic molecule C8H8‚ the eight carbon analogue to benzene. a) Write the secular detrerminant corresponding to the pi-bonding in C8H8. b) Using the secular determinant‚ the following energies are found for the pi-bonding molecular orbitals: 1 = + 2 2 = + 1.41 (two states) 3 = (two states) 4 = - 1.41 (two states) 5 = - 2 Give the electron
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Osmosis The purpose of this lab is to study how membranes of plant and animal cells react when exposed to different solutions. The first experiment involves purple onion skin and the second involves rat blood in various solutions. I needed to understand certain terms before preforming this lab to be able to efficiently explain what is happening to the cells. Diffusion is the tendency of a substance to move down its concentration gradient from a more concentrated to a less concentrated area.
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and associated with organisms are biomolecules. Today in lab‚ a series of tests were ran. Tested were the biomolecules of cells which are lipids‚ sugars‚ proteins and starch. The purpose of these tests were to test for the presences of cellular biomolecules. Some of the things that fat consist of are one glycerol and three fatty acids. Proteins are considered polymers. They are made up of smaller molecules called subunits. Now with proteins they contain a large number of amino acids that are joined
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The team of forensic anthropologists ran a gel electrophoresis with DNA from Skeleton 3 and two missing persons‚ Julia Ly and Teresa Chen to help in DNA identification. This process would allow restriction enzymes to cut by a specific restriction site and run through the gel‚ where the DNA fragments would move from the negative side to the positive side of the gel due to the negative charge of the phosphate group in DNA. The smaller the DNA fragments‚ the further they move down the gel. As mentioned
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