from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method used for the amplification of DNA
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BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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Polypeptides‚ better known as proteins‚ carry out nearly all of a cell’s functions. A protein is made up of at least one or more amino acid polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell
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The main purpose of this lab is to explain what emulsifiers and emulsions are and how emulsifiers affect emulsion stability. An emulsion is a mix of to or more liquids that usually do not mix together. One liquid is evenly dispersed in small droplets throughout the other liquid. Many emulsions contain water as one of the phases. There are two different types of emulsions. One type of emulsion contains oil droplets that are dispersed within water. It is considered oil in water emulsion. The other
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INTRODUCTION The cell membrane consists of mostly phospholipids and proteins which gives the cell its selectively permeable nature. The function and permeability of the cell membrane depends on its whole structure. When destroyed‚ the permeability of the cell membrane is disrupted causing cellular contents to leak out. When cells are cut‚ the cell membranes are mechanically ruptured. High temperature disrupts the structure of proteins and certain chemicals such as acetone and methanol dissolve the phospholipids
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Arielle Fisher GFP Lab November 16‚ 2011 Figure 1 A. B. C. D. Figure 1. Confocal images at 400x magnification of HeLa cells to locate GFP activity. HeLa cells were (A) tagged with Green Fluorescent Protein (GFP) (B) labeled with GFP and 14.13 µl Dexamethasone (C) tagged with GFP fused to the glucocorticoid binding
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In this enzyme lab‚ (insert creative name)‚ the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide
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bacterial proteins to unfold and stick to one another‚ making them nonfunctional
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Enzymes are responsible for multiple reactions that take place naturally in the living organisms. The purpose of the enzymes lab was to investigate how the enzymes play a role in a reaction‚ affecting the rate of reaction (ROR). Interestingly‚ we tested how the enzymes affect the reaction rate at multiple temperatures (0‚ 23‚ 37‚ 50‚ 70‚ and 95 C). It was predicted that an increase in temperature will elevate the thermal activity of substrate which increases the chances the substrate molecules will
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BASICS OF INTEREST RATE SWAPS A swap is an agreement between two institutions to exchange future cash flows. Suppose there is a bank receiving fixed 8% return on 2 year money lent. This bank can swap its revenue stream against another firm’s variable rate 2 year money. In practice swaps tend to be more complicated and can involve more simultaneous variations of interest rates and currency as well. When settled‚ only the differential between the would-be payments are exchanged between two parties
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