OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely as they can either by haemoglobin
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9/4/2014 Fillers within Protein Supplements Local gym enthusiasts are trying to reveal if certain new dietary supplements will actually help them gain muscle mass by providing high levels of protein. Before the users consume the new supplements they want to know if they are indeed true protein supplements or if the manufacturer used fillers as a mean to make more profit. “Do the new supplements contain fillers such as sugar and starch or are they pure protein?” This is important
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1.Carbohydrates Consists of CnH2xOx • Include sugars • Classified into groups based on the number of carbon atoms per molecule. • Classes of Carbohydrates o Monosaccharides ▪ Simple sugars o Disaccharides ▪ Compound sugars o Polysaccharides ▪ Complex sugars a. Monosaccharides • Simple sugars • Contain about 3 to 6 carbon atoms per molecule o Trioses
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EXPERIMENT 1: Proteins -polypeptides compose of > 50 amino acids Functions 1. Enzymes or subunits of enzymes-enhancing the rates of reactions 2. Structural or Mechanical roles 3. Immune response roles 4. Storage and transport of substances 5. Source of amino acids for organisms that cannot synthesize amino acids naturally ISOLATION- disruption of cell membranes to release cell contents; separation for other contaminants 1. CENTRIFUGATION 2. SALTING OUT-water
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Pre-Reading Notes (Very Short Introduction - Chapter 5; Fundamentals of Forensic Science – Chapter 11): • Red and white cell blood antigens are polymorphic: they exist in more than one form and everyone inherits one or more forms of each of them • A successful DNA profile makes it possible to reach a conclusion that a DNA sample came from a specific individual‚ giving law enforcement and forensic science a new‚ powerful identification tool that complements fingerprints and other methods of identification
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things maintain themselves c. Living things have the capacity to reproduce d. Living things are part of populations that remain constant from one generation to the next 2. The macromolecules made from carefully folded strings of amino acids are a. Proteins b. Carbohydrates c. Lipids d. Nucleic acid 3. One difference between prokaryotic and eukaryotic cells is a. Prokaryotic cells have a nucleus‚ whereas eukaryotic cell do not b. Eukaryotic cells have existed on earth far longer than prokaryotic cells
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UNIVERSITY OF MALAWI BUNDA COLLEGE FEEDS AND FEEDING (ANS 321) 2012 LECTURE NOTES. Raphael Woyera Banda (AGR/09/14)‚ Bunda College‚ P.O Box 219‚ Lilongwe. FEEDS AND FEEDING (ANS–321) - 2012. Animals are kept for a purpose and must benefit the one keeping them. The benefit may be; - The animal itself (draft‚ entertainment and prestige) or - The products from the animals for instance milk‚ meat or hides. Animal husbandly involves; - Animal health‚ - Animal breeding
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of the isomerase domain from a spliceosome-correlated cyclophilin‚ PPWD1 which is known as peptidylprolyl isomerase domain and WD repeat containing 1 or peptidylprolyl isomerase containing WD40 repeat‚ has been solved to 1.65 A ˚. PPWD1 acts as a protein-coding gene and its related pathways are mRNA splicing. An important paralog of this gene is PPIL2. This polypeptide encodes an N-terminal WD40 repeat domain as well as a C-terminal domain homologous to Cyps. PPWD1 was previously
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Biology II Step 1: How will you identify the “vital” cellular protein that the virus targets for degradation? (Hint: think proteomics). (3 pts.) First‚ since we know the viral RNA sequence and its targeting protein‚ we can investigate it in bioinformatics database‚ and can acquire some clues or hints about the target proteins. From the bioinformatics database‚ we might be able to find its structure‚ similarity with other proteins‚ functions‚ and binding domains. In other words‚ we can get some
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1. PROTEIN is composed of amino acids. It is required to synthesize and repair muscle and other tissues and maintain fluid and acid-base balance in the body. Proteins also function as enzymes‚ hormones‚ and transporters. If necessary‚ the body uses protein to meet energy needs (4 kcals per gram). Excess kcals from protein are stored as body fat. If you consumed 60 grams of protein in a day‚ how many kcals would this convert to? (Choose your answer and delete the rest). (1 point) b)
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