1. Define the terms anatomy and physiology. Explain the principle of complementarity and how it applies to this course. The term anatomy refers to the structure of the body and its parts. Along with the study of the body structure‚ anatomy also refers to how these body parts work together and their working relationship as a whole. Physiology is the study of how the living systems in the body function and work. When studying the Physiology of the body it is most understandable if terms from the underlying
Premium Endoplasmic reticulum Feedback Negative feedback
cell/ movement within cell. Microfilaments‚ microtubules‚ intermediate filaments Ribosomes (non membrane bound) Protein synthesis‚ catalyzes RNA reactions Nucleus (membrane bound) nuclear envelope‚ nuclear pores‚ nucleolus‚ DNA/ proteins organized into chromosomes/ chromatin Endoplasmic Reticulum (membrane bound) Tubular membranes and cisternae‚ Rough= works with ribosomes on protein synthesis‚ Smooth=
Premium Cell membrane Protein Signal transduction
SDS-PAGE is to separate peptide chains in a protein sample according to molecular weight by sieving them through a gel matrix under the influence of an electric current. The setup for an SDS-PAGE experiment is shown in Figure 1.0 [pic] Figure 1.0- An illustration of a simple SDS-PAGE setup Based on the figure above it can be observed that big proteins (high MW) travel a shorter distance than that of smaller proteins (low MW). This is because bigger proteins have a difficult time in traversing through
Premium Protein Enzyme Molecular biology
the migration of proteins (ex: 4% gel vs. 18% gel)? 2. [8pts]Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. [45pts]You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-PAGE analysis. 1 2 3 4 Figure 1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons)
Free Gel electrophoresis Cell Gel
myth that it is best to double or triple your protein intake during weight training. In fact‚ fitness experts don’t agree on just how much proteins an individual should
Premium Nutrition Obesity Metabolism
expressions of P-glycoprotein (a multidrug transport protein) and multidrug resistance-associated protein (MRP-1); increased levels of the cellular detoxification protein‚ glutathione; and changes in the expression of apoptosis associated proteins such as Bcl-2‚ FasL and p53‚ which generally results in promoting cell survival [1‚2‚3‚4]. P-glycoprotein expression is the mechanism mostly associated with classical multidrug resistance (MDR)‚ as this protein is responsible for the active transport of various
Premium Apoptosis Oncology Cancer
It must be supplied by the diet. It can be made from fat in the body. It can be made from glucose in the body. Question 3 of 30 Which of the following describes a process in protein synthesis? The code to make a protein is carried by a strand of messenger RNA. The final step in completing the protein is carried out in the mitochondria. The function of transfer RNA is to assist in absorption of amino acids into the cell. The DNA binds to ribosomes and directs uptake of specific
Premium Protein Metabolism Nutrition
classic first step to fractionate proteins by causing perturbations in the solvent with respect to ionic strength. Historically‚ separation methods were limited and as a result precipitation methods were highly used with very fine cuts in ppt conditions. As more choices of inexpensive and quality resins are commercially available precipitation steps are typically limited to one or two initial cuts in the beginning of purification or simply used to concentrate the proteins. The major advantage to (NH4)2SO4
Premium Solubility Precipitation Protein
Polymers-the largest of the macromolecules. These are composed of numerous‚ small identical subunits known as Monomers. There are 4 major polymers that are important for living organisms. These polymers are; carbohydrates‚ lipids‚ proteins and nucleic acids. B. Polymers are referred to as being organic compounds. Organic compounds are compounds that contain the elements carbon and hydrogen. 1. Chemical Properties of Carbon a. Carbon has 6 electrons. What is the structure
Premium Amino acid Protein DNA
Protein purification From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function‚ structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines
Premium Protein Chromatography