Materials and Methods: Methods and materials used in this experiment can be found in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf Value Color A 6.0 57.0 0.22 Bluish green B 17.5 56.0 0.105 Dark green blue C 4.0 57.0 0.070 Greenish blue D 4.0 47.0 0.085 Green E 10.5 56.5 0.186 Light blue Figure 1: Chromatography- TLC sheet From left to right- acetic
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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EE314: CMOS RF Integrated Circuit Design Introduction to Wireless Communication systems Stanford University Hamid Rategh Hamid Rategh Stanford University EE314 HO#1 1 Course Staff Instructor: Dr. Hamid Rategh Email: hamid@smirc.stanford.edu Office Hours: MW 2:15-3:15PM @ CIS-126; Phone: 725-8313 TA: Mehdi Jahanbakht and Deji Akinwande Email: ta314@smirc.stanford.edu Office Hours @ Packard 106 Sunday 3:00 – 3:30 pm (for SCPD students only) Sunday 3:30 – 5:00 pm Thursday 5:00 –
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Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
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Communication styles a culture’s preferred ways of communication‚ given its core assumptions and norms.. Types of communication styles 1. Clear and Direct Clear and direct communication is the most healthy form of communication and occurs when the message is stated plainly and directly to the appropriate family member. An example of this style of communication is when a father‚ disappointed about his son failing to complete his chore‚ states‚ “Son‚ I’m disappointed that you forgot
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Gel filtration GEL FILTRATION OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely
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PROTEIN TRANSLATION 1. Initiation Translation To begin translation: large and small ribosomal subunit‚ along with the initiating tRNA‚ assembles onto the mRNA 2. Translation Elongation The next amino acyl-tRNA enters the ribosome at the A site The amino acid at the P site is transferred to the tRNA at the A site. The ribosome moves one codon farther along the mRNA‚ releasing the empty tRNA through the E (exit) site. The A site is free for the next incoming RNA. The cycle is repeated as the
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Marissa Arias Senior Rhetoric Graf-8 April 14‚ 2014 Gel II Manicure Many women and even some men love to get their nails done‚ but hate how much time it takes to dry and on top of that they are extremely easy to ruin and chip. There is a new type of manicure trending know as Shellac‚ Gel nails‚ or No chip. According to my cosmetology instructor Samantha Rudman‚ this process takes about 35 to 40 minutes but dries in seconds. Many individuals would love to know this process even the men that
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and that is where other techniques are considered to treat pain that just will not go away with traditional pain relievers. Many doctors perform a procedure called Radiofrequency Ablasion (RFA) to minimize the nerves that signal the pain receptors. Rf Ablasion Edmond is used to help those who suffer from chronic neck and back pain find relief without an invasive surgical procedure that can mean long recovery times. Doctors perform ablasion therapy to benefit their patients‚ sparing them the discomforts
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and Engineering‚ NMIMS A Report On RF Network Planning For GSM and CDMA By Kashish Parikh UNDER THE GUIDANCE OF Prof Ravindra Bhatt(Lecturer)‚ Parminder Singh Sodhi(Dept Gen Manager) and Vivek Porwal(Junior Manager) Reliance Communications (8th May 2010) SVKM’s Narsee Monjee Mukesh Patel School of Technology Management and Engineering‚ NMIMS Certificate This is to certify that Kashish Parikh has submitted his report on RF Network planning under the guidance of Mr
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