Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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the gel after electrophoresis. Gel 1 Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis
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How Much Protein Do You Really Need? Why is protein important? Protein is part of an important food group that you will need in order for your body to be able to function properly. As a macronutrient‚ protein allows your body to undergo proper growth and development‚ as well as to strengthen its immunity against various sicknesses and diseases. Protein is also responsible for acting as the main building block that repairs your tissues‚ organs‚ tendons‚ muscles‚ and even your bones‚ skin‚ and eyes
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1. INTRODUCTION The project “Automated Electricity Meter” aims to move away from the traditional method of manual reading of electricity meters in which an individual has to physically record the reading. Instead the project proposes to successfully be able to take the meter reading automatically without having a person be physically present while taking the reading‚ thereby reducing manpower requirement. This system helps the users by alerting them about the peak loads (max energy consumption)
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Question 5: The chromophore in this assay is Coomassie Brilliant Blue dye. Question 6: It is important to set up a blank to separate the solute (saline) from the protein (stock). By subtracting the absorbance of the blank (which has no protein present) from the original absorbance the absorbance of the protein at each concentration will remain. Question 7: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds‚ forming reduced
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Protein Synthesis Worksheet 1. 2. a. Protein synthesis began in the nucleus. b. Chromatin is a diffuse mass located in the nucleus where genetic material is found. c. A gene is a sequence of nucleotides‚ it is important because they form the template and these templates are used for synthesizing proteins. 3. a. Transcription is the first step of protein synthesis it uses a sequence of DNA nucleotides to produce a sequence of RNA nucleotide. b. RNA polymerase bonds to RNA nucleotides together
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Why are proteins important? Proteins can easily be addressed as one of the most important molecules in animal cells. There are many varieties of protein molecules which allow a vast range of biological activities in the cell.( Williams‚ G.(2000))’Proteins are big compound with large molecular masses’’(Williams‚ G.(2000)) whose main chemical constituents are hydrogen‚ carbon ‚oxygen and nitrogen. A protein structure consists of a specific sequence of amino acids called the
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Protein Article Research Sara Langrell December 15‚ 2011 Nutrition SCI/241 Dr. Venessa Lee Abstract: Athletes have been searching for years for a way to reduce the amount of recovery time between work outs. Based on this issue there has been quite a lot of research conducted to find out what can be done‚ if anything‚ to either reduce or eliminate recovery time. One of the theories is to increase protein intake above the daily recommended amount‚ thereby providing the body with additional
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are present in a protein‚ in this case gluten. Introduction: Proteins are large biological molecules that are made up of amino acids. They serve as building blocks that form organic structures such as tendons‚ muscle‚ and cartilage. Enzymes - biological catalysts that allow important chemical reactions to occur in our body - are also made up of proteins. Thus‚ proteins play a fundamental role in the processes of life. [1] Protein purification is basically isolating a protein from complex mixtures
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the ideal body weight. A perfect choice of foods and the exact post workout protein can be a perfect combination in keeping a healthy torso. It is very important to choose the right kind of foods to provide the body with the required energy. The foods that an individual must ingest following an exercise are definitely distinctive than the food being consumed on a regular basis. Ingesting foods and a post workout protein is crucial because the body is reduced with the essential vitamins and
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