in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe that both types of treated cells will express the beta-actin gene because it is a housekeeping gene. However‚ only HL-60 cells treated with PMA will express the MMP-9 gene by their RNA. The purpose of this lab is to view the effects of differentiation on protein synthesis
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High-Protein Diets Christine J. Williams SCI 241 September 19‚ 2012 Diane Carson High-Protein Diets Proteins are a vital nutrient that must be provided by one’s diet. Bones‚ skin‚ muscle‚ hair‚ and most other tissues are made up of proteins. The continuous replenishment of new protein from one’s diet keeps the body functioning properly. When a person is consuming a high protein diet‚ it is inevitable to be on a low carbohydrate diet as well. There are a lot of foods that contain protein and
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High Protein Diets: Good or Bad? Lose weight while feasting on steak‚ burgers‚ cheese‚ and bacon? All without feeling hungry? What’s not to love? Meat lovers have flocked to high-protein‚ low-carb plans like Atkins‚ Zone‚ Protein Power‚ and Sugar Busters. While these diets can work‚ you need to carefully consider the risks and rewards before deciding if one is right for you. How Much Protein? Most Americans get 12%-18% of their calories from protein. With a high-protein diet‚ it can be much
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Bernardine Date Due: 02/08/2013 PURISIMA‚ Dio Mark Angelo Date Submitted: 02/08/2013 Experiment No. 9 AGAROSE GEL ELECTROPHORESIS OF DNA Abstract _____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform
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B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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PROTEIN ENERGY MALNUTRION Types include:[2] * Kwashiorkor (protein malnutrition predominant) * Marasmus (deficiency in both calorie and protein nutrition) * Marasmic Kwashiorkor (marked protein deficiency and marked calorie insufficiency signs present‚ sometimes referred to as the most severe form of malnutrition) Note that this may also be secondary to other conditions such as chronic renal disease[3] or cancer cachexia[4] in which protein energy wasting may occur. Protein-energy
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Short Description Protein Dynamix has revolutionised the sports supplement industry‚ with its range of best sports nutrition products. The company aims to help people accomplish their fitness goals without costing them an arm and a leg. From effective fat loss supplements to superior quality protein and many others‚ whether you want to get in shape‚ lean your muscle or manage your weight‚ the shop offers only “best in class” products from leading nutrition brands‚ so to make sure you only get quality
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17.1 Genes specify proteins via transcription and translation * George Beadle and Edward Tatum worked together with mutated (Neurospora crass) bread mold to figure out that they were missing a specific enzyme (gene) that catalyzed and synthesized a pathway required. They concluded that they were missing that enzyme because it was lacking the amino acid that coded for the enzyme‚ thus was mutated and incapable of growing. Led to the one enzyme-one gene hypothesis. The Products of Gene Expression:
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where the protein was fully denatured. Then‚ the curve dropped quickly. With the raising of the temperature‚ protein in the solution got denatured. When the temperature increased from 60℃ to 80℃‚ the structure of protein got unfold. When the solution was heated‚ the H-bond was broken. The hydrophobic parts of protein were inside the protein surrounded by hydrophilic parts before unfolding. With the temperature increasing‚ the hydrophobic parts were exposed. The hydrophobic parts of proteins interacted
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Give an account of protein structure and function Protein structure 1 Proteins consist of amino acids joined together (in chains) 2 A protein is unique because of the sequence of amino acids 3 The amino acids are joined by strong peptide bonds 4 to produce the primary structure 5 Further (weak) hydrogen bonding between acids 6 produce the secondary and tertiary structures A maximum of 4 marks can be gained from this section. Protein function 7 Some proteins are enzymes + named example
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