Macromolecular Composition of the Liver Cell Formal Report Aims The aim of the experiment was to test for the presence of DNA‚ RNA‚ protein and glycogen in the cytoplasm and the nucleus of bovine liver cells. From the findings of the results the distribution of these macromolecules can be shown within the liver cell. This was carried out by undertaking qualitative experiments‚ where the observation of a colour change was noted and a quantitative experiment‚ where numerical data was recorded from
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1 Grow Taller 4 Idiots 2 Copyright ©2009 by GrowTaller4Idiot.com All rights reserved. No part of this digital book shall be reproduced‚ stored in a retrieval system‚ or transmitted by any means‚ electronic‚ mechanical‚ photocopying‚ recording‚ or otherwise‚ without written permission from the publisher. No patent liability is assumed with respect to the use of the information contained herein. Although every precaution has been taken in the preparation of this digital book‚ the publisher
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study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that
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Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods. 2
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An ABC transporter is system that involves the use of a substrate binding protein and ATP. By combining these the substrate binding protein and ATP‚ the transporter is able to increase the concentration of the nutrient inside the cell‚ even if it is against the concentration gradient. When comparing the concentration of nutrient‚ the cytosol often has a higher concentration of nutrients compared to the periplasm and‚ possibly the environment (depending on how nutrient rich the environment is. For
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Graphical Analysis of the Structure of the Ubiquitin Protein Molecule Using the VMD Computer Program Author’s Name Chemical Engineering Department‚ University of New Hampshire - Durham ABSTRACT VMD is a molecular visualization program developed by the Theoretical and Computational Biophysics group of the University of Illinois at Urbana-Champaign with the goal of displaying‚ animating‚ and analyzing large biomolecular systems using 3-D graphics and built-in scripting. Therefore‚ The VMD program
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Transcription and Translation Notes for IB Diploma Year 1 DNA is a huge information database that carries the complete set of instructions for making all the proteins a cell will ever need! Although there are only four different bases in DNA (A‚ C‚ G and T)‚ the order in which the bases occur determines the information to make a protein‚ just like the 26 letters of the alphabet combine to form words and sentences: Compare: RAT - TAR - ART - same 3 letters; completely different meanings. And
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Miriam Yanez Physics 018-01 Literature Review 25 April 2012 Biophysics and protein dynamics have shaped our lives when it comes down to advancements in medicine. In living cells‚ proteins are the main components that keep the organism running at its best with every single protein and enzyme having their own function. The discovery of DNA‚ RNA‚ and other protein synthesis became the structures of life in a new perspective in the smallest ways leading to the continuing studies to uncover more
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SUMMARIES AT END OF CHAPTERS UNIT 1 Macromolecules * Large molecules sometimes composed of a large number of repeating subunits * Four major classes: Carbohydrates (made of simple sugar/glucose)‚ Lipids (Glycerol and Fatty Acids)‚ Proteins (Amino Acids) and Nucleic Acids (Nucleotides) * Condensation reaction/dehydration synthesis: a reaction that creates a covalent bond between two interacting subunits. Removal of H from one functional group and an OH from the other‚ and the H
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mechanism that involve restricting proton entry by cytoplasmic membrane and purging of protons and their effect by cytoplasm. pH homeostatic mechanisms The cell membrane is highly impermeable to protons Membrane channel have a reduced pore size. Protein influx inhibited by chemiosmotic gradient Excess proton pumped out of the cell Cytoplasmic buffering helps to maintain the intracellular pH 1. The cell membrane is highly impermeable to protons High impermeable cell membrane to restrict proton
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