botanist regarding the daisy-like plants she had found. The botanist told the investigating agent that the daisies might represent different species. Specifically‚ the agent was advised that the size of the “compositase” protein could be a clue to the identity of the plants. Proteins from all three
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(PTS2). In a normal cell‚ the Pex7p receptor has a PTS2 receptor region that recognizes and binds the PTS2 of the localized protein in the cytosol‚ necessary for transport into the peroxisome (Braverman et al. 1993). Pex7p receptor works in conjunction with Pex5p receptor‚ which equivalently recognizes peroxisome-targeting signal 1 (PTS1) on the C-terminal domain of proteins. Once the mobile cytosolic import
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gap in-between which because it resembles a channel and shows the passage way of the protein. (D) Carrier Protein – facilitated diffusion of glucose. The carrier protein transports large molecules like amino acids and glucose from high concentration to low concentration. We used modeling clay with a large gap to show the large molecules are transported between through the carrier protein. (E) Receptor Protein and Signal Molecule-**Functional** These items allow cell to communicate with other cells
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1. Define the terms anatomy and physiology. Explain the principle of complementarity and how it applies to this course. The term anatomy refers to the structure of the body and its parts. Along with the study of the body structure‚ anatomy also refers to how these body parts work together and their working relationship as a whole. Physiology is the study of how the living systems in the body function and work. When studying the Physiology of the body it is most understandable if terms from the underlying
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cell/ movement within cell. Microfilaments‚ microtubules‚ intermediate filaments Ribosomes (non membrane bound) Protein synthesis‚ catalyzes RNA reactions Nucleus (membrane bound) nuclear envelope‚ nuclear pores‚ nucleolus‚ DNA/ proteins organized into chromosomes/ chromatin Endoplasmic Reticulum (membrane bound) Tubular membranes and cisternae‚ Rough= works with ribosomes on protein synthesis‚ Smooth=
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SDS-PAGE is to separate peptide chains in a protein sample according to molecular weight by sieving them through a gel matrix under the influence of an electric current. The setup for an SDS-PAGE experiment is shown in Figure 1.0 [pic] Figure 1.0- An illustration of a simple SDS-PAGE setup Based on the figure above it can be observed that big proteins (high MW) travel a shorter distance than that of smaller proteins (low MW). This is because bigger proteins have a difficult time in traversing through
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BIOMOLECULES 1.What are macromolecules? Give examples. Macromolecules are large complex molecules that occur in colloidal state in intercellular fluid. They are formed by the polymerization of low molecular weight micromolecules. Polysaccharides‚ proteins‚ and nucleic acids are common examples of macromolecules. 2. Illustrate a glycosidic‚ peptide and a phospho-diester bond. (a) Glycosidic bond is formed normally between carbon atoms‚ 1 and 4‚ of neighbouring monosaccharide units. (b) Peptide
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the migration of proteins (ex: 4% gel vs. 18% gel)? 2. [8pts]Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. [45pts]You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-PAGE analysis. 1 2 3 4 Figure 1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons)
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myth that it is best to double or triple your protein intake during weight training. In fact‚ fitness experts don’t agree on just how much proteins an individual should
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expressions of P-glycoprotein (a multidrug transport protein) and multidrug resistance-associated protein (MRP-1); increased levels of the cellular detoxification protein‚ glutathione; and changes in the expression of apoptosis associated proteins such as Bcl-2‚ FasL and p53‚ which generally results in promoting cell survival [1‚2‚3‚4]. P-glycoprotein expression is the mechanism mostly associated with classical multidrug resistance (MDR)‚ as this protein is responsible for the active transport of various
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