separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at different rates. Hypothesis: If chlorophyll is removed from the plant‚ then the different pigments in the plant can be visible. Materials: -Spinach -Coin -Chromatography paper -Ruler
Premium Chlorophyll Photosynthesis
Introduction ESTERS are compounds derived from the reaction of a organic acid with an alcohol. Acid + Phenol/alcohol --> ester + water R--C=O + R’--OH ----> R--C=O + H2O | | C-OH C-O-R’ Esters are the compounds that give fruits their characteristic flavours and odours. ie. methyl salycilate is "Oil of Wintergreen". http://www.petrik.com/PUBLIC/library/misc/glossary_of_org_chem.htm#ESTERS Aim To synthesise the ester 3-methylbutyl ethanoate( isopentyl acetate) and to identify the product
Premium Acetic acid Ammonia Ester
Christy Chan (5) BIOLOGY LAB REPORT Investigation of the effect of metal ions on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates
Premium Enzyme Silver
Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
Premium Thin layer chromatography Chromatography Color
Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to
Premium Gas chromatography Chromatography Gas
Candy Chromatography Background info: Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water‚ separation of plant pigmentation‚ and analysis of narcotics. Source: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b Purpose: To find out why candies are different colors. * Materials: Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
Premium Color Water Ethanol
The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
Premium DNA Bacteria Escherichia coli
Protein Essay- Erin Keegan What are proteins? A polypeptide is a chain of monomers joined up to make a polymer. Proteins are organic polypeptides made up of a chain of amino acid residues- they’re residues because they lose a water molecule. The amino acids (monomers) join up by forming peptide bonds. When two amino acids join together‚ they become a dipeptide. This happens when the carboxyl group of an amino acid and the amino group of another amino acid link up through a condensation reaction (which
Premium Protein Amino acid
from the exchange of positive and negative ions between reacting solutions of two ionic compounds. Thus‚ in this example the precipitate must be either silver chloride‚ AgCl or sodium nitrate‚ NaNO3. Sodium nitrate dissolves readily in water and is therefore soluble. Thus we can conclude that silver chloride is the insoluble precipitate. We can represent the formation of this precipitate by a net ionic equation: Ag+ +NO3 ̄ +Na++Cl ̄→AgCl+NO3 ̄+Na+ Net: Ag+ (aq)+Cl ̄ (aq)→AgCl(s) Ions present in
Premium Solubility Ion Chemistry
ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning
Premium Dye Food coloring Analytical chemistry