Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary‚ secondary‚ tertiary‚ and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from
Premium Protein Amino acid
Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
Premium Protein
Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
Premium Thin layer chromatography Chromatography
Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
Premium Enzyme Chromatography Analytical chemistry
of a functional recombinant fusion protein via the directional sub-cloning of an E.coli derived tyrosine phosphatase gene (wzb) into a pT5(6H)CFP mutant expression vector. Abstract: Application of fluorescent fusion proteins to the field of expression and interaction proteomics as a means of dynamic imaging proteins in vivo has allowed for rapid advancements in biotechnology research. Production of such proteins first involves the insertion of a given protein-coding gene transcriptionally in-frame
Free Protein DNA
Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
Premium Protein Amino acid Acid
Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
Premium Protein Cell DNA
TITLE: The Amount of Protein in Chicken Tissues over Cooked Various Periods of Time. ABSTRACT: In this lab‚ we are using a BioRad protein assay dye to determine the concentration of protein in our chicken. The dye binds to the amino acid residues‚ which allow us to find the concentration of protein (BioRad Protein Assay for Tissues). Our hypothesis was the longer chicken is cooked the less protein is available. To test our hypothesis‚ we made samples using our chicken and distilled water to determine
Premium Amino acid Water Acid
Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
Premium Chromatography Solubility Gas
Reactivity of Halide Ions Purpose: There are four halide salts used in this experiment that are found in the human body. Sodium fluoride is poisonous‚ but has been traced to be beneficial to humans in the prevention of tooth decay. Sodium chloride is added to many of our foods to increase flavor. Sodium chloride is important for many life processes‚ but too much intake is linked to high blood pressure. Sodium bromide is distributed throughout body tissues. Sodium iodine controls cell growth. The
Premium Sodium chloride Chlorine Iodine