com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment‚ liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First‚ the dyes responsible for the purple color‚ FD&C Blue #1 and Red #40 are separated. Then‚ in a second experiment‚ the other components of Kool-Aid®‚ the flavorings and citric acid‚ are separated as well. Concepts • Resolution • Liquid chromatography • Selectivity
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In paper chromatography‚ some compounds in a mixture travel almost as far as the solvent does; some stay much closer to the base line. The distance travelled relative to the solvent is a constant for a particular compound as long as you keep everything else constant. Some compounds may travel farther than others because they may be composed of more components‚ thus traveling farther. For example‚ black is composed of every color of the rainbow‚ while yellow is composed of less colors. As a result
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Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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Objective: Separate and purify acetanilide from a mixture by recrystallization. Compare the melting points of crude and recrystallized acetanilide. Introduction : The most common method of purifying solid organic compounds is by recrystallization. In this technique‚ an impure solid compound is dissolved in a solvent and then allowed to slowly crystallize out as the solution cools. As the compound crystallizes from the solution‚ the molecules of the other compounds dissolved
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Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the components of an unknown solution
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entire organism. Bones are constantly breaking down and rebuilding‚ and calcium is directly involved in this process. Calcium ions (Ca2+) are essential mediators of a wide variety of cellular activities‚ including the passing of information along neurons and between adjacent neurons. Hormonal regulation is also mediated by the proper functions of calcium and calcium ions in the body. Considering all of the roles that it plays‚ calcium is of the upmost physiological importance and is the most abundant
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are present in a protein‚ in this case gluten. Introduction: Proteins are large biological molecules that are made up of amino acids. They serve as building blocks that form organic structures such as tendons‚ muscle‚ and cartilage. Enzymes - biological catalysts that allow important chemical reactions to occur in our body - are also made up of proteins. Thus‚ proteins play a fundamental role in the processes of life. [1] Protein purification is basically isolating a protein from complex mixtures
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Exchange is change. Rapid‚ brutal‚ beautiful‚ hurtful‚ colorful‚ amazing‚ unexpected‚ overwhelming and most of all constant change. Change in lifestyle‚ country‚ language‚ friends‚ parents‚ houses‚ school‚ simply everything. Exchange is learning to trust. Trust people‚ who‚ at first‚ are only names on a piece of paper‚ trust that they want the best for you‚ that they care. Trust‚ that you have the strength to endure a year on your own‚ endure a year of being apart from everything that mattered to
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Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used
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unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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