Anionic Exchange Ion Chromatography to Detect the Anions Concentrations in the water Samples Narendra Boppana‚ Nasser Ali‚ Martin Chavez‚ Sear Introduction: Ion Exchange Chromatography is a process where the ionic exchange occurs in between the stationary phase and the sample in the mobile phase‚ by using this ionic exchange phenomenon it is easy to separate the compounds. Ionic exchange depends on the electrical properties
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monomeric subunits of proteins‚ enzymes‚ and peptides in biological systems. Amino acids contain a carboxylic acid group‚ an amine group‚ and a unique side chain‚ which determines its properties. There are more than 500 amino acids; however‚ only 20 are commonly found1. Due to their importance in biological systems‚ it is important to be able to experimentally analyze and isolate amino acids by separation techniques. One such method of separation is thin layer chromatography (TLC). In this method
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Determination of Percent Potassium & Percent Iron in an Iron Oxalate Salt by Ion Exchange Introduction: This experiment involves determining both the percent potassium (K) and iron (Fe) in a single titration after passing a solution containing a known mass of complex salt through an ion exchange column. Ion Exchange: Certain materials called ion exchange resins consist of rather large molecules which contain ions that can be displaced. The resins are solids‚ insoluble in water‚ usually granular
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for the purification of proteins What are proteins? Proteins are the main building blocks of life. They are essential for the body and have many different roles . Proteins are made from sequence of amino acids. Protein structure is determined by their sequence of amino acids‚ which are linked by peptide link. Proteins are made from about 50 to 2000 amino acid residues. Figure 1 Why purify proteins? They are many reasons why a biochemist might want to purify a protein. For example
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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Protein purification From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function‚ structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines
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In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system‚ thus separating them. Chromatography is necessary in chemical industries‚ as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler forms)
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healing and recovery especially in severely wounded patients. One of the approaches for treating wounds is the use of biocompatible composites incorporated with antibacterial agent to improve wound healing and diminish the wound infection risk 2. Ion-exchange zeolite has gained attention due to its renewable nature‚ good biocompatibility and excellent
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What is water purification? Water purification generally means freeing water from any kind of impurity it contains‚ such as contaminants or micro organisms. Water purification is not a very one-sided process; the purification process contains many steps. The steps that need to be progressed depend on the kind of impurities that are found in the water. This can differ very much for different types of water. In which ways is polluted water treated? Settling Before the purification process begins some
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