Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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monitor the binding if s substrate to a protein. The substrate can give a very different CD spectrum when free in the solution relative to when bond in solution. Outside of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed
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Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Water purification is the process of removing undesirable chemicals‚ biological contaminants‚ suspended solids and gases from contaminated water. The goal is to produce water fit for a specific purpose. Most water is purified for human consumption (drinking water)‚ but water purification may also be designed for a variety of other purposes‚ including meeting the requirements of medical‚ pharmacological‚ chemical and industrial applications. In general the methods used include physical processes such
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acid. Observation Solutions smells like vinegar. Deduction Solution contains CH3COO- ions. A white precipitate is formed. Solution turns cloudy and a white precipitate is formed. Solution contains either Pb2+ ions‚ Zn2+ ions or Al3+ ions. Solution contains either Pb2+ ions or Al3+ ions. Solution contains CH3COO- ions. Solution contains CH3COO- ions. Solution contains Pb2+ ions. Solution contains Pb2+ ions. Solution turns brown and a white precipitate is formed. A white precipitate is formed
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Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation of chemical mixtures
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DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
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