"Protein purification via ion exchange chromatography" Essays and Research Papers

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    Solar Purification

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    “Solar Treated Purification” Intorduction: A. Background of the study: In our world today‚ purified water is the one that most of the people drink everyday especially those who live in cities. Water purification is the process of removing undesirable chemicals‚ biological contaminants‚ suspended solids and gases from contaminated water. The goal is to produce water fit for a specific purpose. Most water is purified for human consumption (drinking water)‚ but water purification may also be designed

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    Purification of Solids

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    Purification of solids Aim: To separate a mixture of carboxylic acid and a neutral substance by treatment with aqueous sodium hydroxide and purifying the carboxylic acid‚ measuring the melting points of the neutral and the acid components. METHOD: A mixture of( 5g) Carboxylic acid and neutral compound were separated by adding 2 mol dm-3 sodium hydroxide (25cm3) to the mixture‚ which separated the water soluble sodium from the water insoluble neutral component‚ by filtration. The solid at the

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    Protein SCI/241 Protein A protein are organic compounds that made up of amino acids and are the building blocks of the cells in the body. Every cell in the body requires proteins to grow and repair themselves so these proteins are necessary for a healthy body and survival. Having so much protein can hurt your body so when you consume so much can get you sick and the right size of protein can keep you from getting sick. They’re five types of protein in everything that we eat. The largest class

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    Protein

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    PROTEIN TRANSLATION 1. Initiation Translation To begin translation: large and small ribosomal subunit‚ along with the initiating tRNA‚ assembles onto the mRNA 2. Translation Elongation The next amino acyl-tRNA enters the ribosome at the A site The amino acid at the P site is transferred to the tRNA at the A site. The ribosome moves one codon farther along the mRNA‚ releasing the empty tRNA through the E (exit) site. The A site is free for the next incoming RNA. The cycle is repeated as the

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    after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each stage were recorded. The volume could be seen in figure 2. The volume of protein decreased in

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    Protein

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    Effect of Protein Intake on Strength‚ Body Composition and Endocrine Changes in Strength/Power Athletes Introduction: Proteins are often called the building blocks of the body. Protein consists of combinations of structures called amino acids that combine in various ways to make muscles‚ bone‚ tendons‚ skin‚ hair‚ and other tissues. They serve other functions as well including nutrient transportation and enzyme production. Adequate‚ regular protein intake is essential because it isn’t easily

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    Chromatography Lab

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    Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the

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    PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine

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    Purification of Water

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    Purification of Water Domestic: Distillation - In the process of distillation water is heated and evaporation takes place‚ whereby water changes back to water when cooled; this process is called condensation. The condensed water is the purest form of water‚ free from microbes‚ and impurities. Boiling - Boiling is the best and simplest method to purify water. After straining the water twice through clean muslin cloth‚ it is boiled for ten minutes lo kill the microbes present in it and also remove

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    Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four

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