"Protein purification via ion exchange chromatography" Essays and Research Papers

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    Purification of a Mixture

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    Assignment Purification of a Mixture INTRODUCTION Mixtures obtained from the biosphere‚ lithosphere‚ hydrosphere or atmosphere can be industrially separated into their constituents. Petrol for example is obtained from crude oil found in the lithosphere. RESEARCH SKILLS When researching this assignment you will access a variety of secondary resources including web sites and textbooks. Please include a detailed bibliography You should also consider both the validity and reliability of your

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    Column Chromatography

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    Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the

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    Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical

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    oil purification

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    OIL PURIFICATION A SCIENCE INVESTIGATORY PROJECT CHAPTER I Background of the Study INTRODUCTION Oil used in cooking foods is commonly derived from vegetables. Cooking oil is commonly used for frying and oil used for frying is usually thrown away. The recycling of cooking oil also provides a form of revenue for restaurants‚ which are sometimes compensated by cooking oil recyclers for their used deep fryer oil. Cooking oil recycling also results in less used oil being disposed

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    Proteins

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    Proteins Are the basic materials of every body cell. Body cells are constantly wearing out. As a result‚ they are continuously in need of replacement Of the six nutrients group‚ only proteins can make new cells rebuild tissue. By the age of 4 years‚ body protein content reaches the adult level of about 18% of body weight. An adequate supply of proteins in the daily diet is essential for the maintenance of health. The word protein is a Greek derivation and means “of first importance”. Composition

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    Protein

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    Introduction: Proteins are necklaces of amino acids – long chains molecules. Proteins are the basis of how biology get this done. As enzymes‚ they are the driving force behind all the biochemical reactions which make biology work. As structural elements‚ they are main constituents of our bones‚ muscles‚ hair‚ skin‚ and blood vessels. As antibodies‚ they recognize invading elements and allow the immune system to get rid of the unwanted invaders. For these reasons‚ scientists have sequenced the

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    Abstract The activity of invertase and the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved

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    Paper Chromatography

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    Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also

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    Nitration and Purification of Bromobenzene Abstract: An electrophilic aromatic substitution reaction was performed on bromobenzene with nitric acid producing both 2-bromonitrobenzene and 4-bromonitrobenzene. Products of the reaction were purified through multiple recrystallizations and column chromatography creating multiple crops of a yellow powder. The percent yield of products was determined to be 51%. The melting point of Crop 1 was found to be 110-115 °C‚ and Crop 2 was found to be 37-90

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    Ion Analysis

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    Testing for Ions Analysis Chemical Changes of Test Solutions Test Solution Silver Nitrate Barium Chloride Potassium Thiocyanate Sodium Sulfate - Stayed white in color - Turned cloudy in color - Precipitation formed in solution - Stayed white in color Potassium Chloride - Turned cloudy in color - Precipitation formed in solution - Stayed white in color - Stayed white in color Iron (III) Nitrate - Stayed orange in color - Stayed orange in color - Changed color from orange

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