"Protein purification via ion exchange chromatography" Essays and Research Papers

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    Proteins

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    BASICS OF INTEREST RATE SWAPS A swap is an agreement between two institutions to exchange future cash flows. Suppose there is a bank receiving fixed 8% return on 2 year money lent. This bank can swap its revenue stream against another firm’s variable rate 2 year money. In practice swaps tend to be more complicated and can involve more simultaneous variations of interest rates and currency as well. When settled‚ only the differential between the would-be payments are exchanged between two parties

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    Metallic Ions

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    Ocean County College Department of Chemistry Identification of Metallic Ions Date Submitted: July 4‚ 2015 Date Performed: July 4‚ 2015 Lab Section: Chem-181DL1 Course Instructor: Prof. Amal Bassa Purpose The purpose of this experiment was to perform and observe the flame tests of some alkali and alkaline earth metal ions. Procedure To begin I placed approximately 0.5 mL of sodium nitrate in a well of the 24-well plate. I then placed approximately 0.5 mL of the remaining

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    Candy Chromatography

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    Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color

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    contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification Maintain the PCR volume of 50µL-100 µL. Pipet the elution buffer in the center of the PureLink® Spin Column (PSC) and perform an incubation. Add B2 to PCR reaction. Add this sample

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    paper chromatography

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    Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and

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    Preparation and purification of acetylsalicylic acid Results and Data treatment (A) Preparation of aspirin i) Details about the reactants Reaction of the acetylation of salicylic acid is following From the balanced reaction above‚ it can be seen that the stoichiometry between salicylic acid and acetic anhydride is 1: 1. In this experiment‚ 21.7mmol of salicylic acid was used to react 6.0mL of acetic anhydride and salicylic acid was limiting reagent. The expected amount of salicylic

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    Gas Chromatography

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    How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component

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    Chromatography Lab

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    Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation

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    Denaturation of Proteins

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    “Denaturation of Proteins” Denaturation is a process in which proteins or nucleic acids lose the tertiary structure and secondary structure which is present in their native state‚ by application of some external stress or compound such as a strong acid or base‚ a concentrated inorganic salt‚ an organic solvent (e.g.‚ alcohol or chloroform)‚ or heat. If proteins in a living cell are denatured‚ this results in disruption of cell activity and possibly cell death. Denatured proteins can exhibit

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    Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for

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