Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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unable to cleave the signal sequence amino acid. B. Band B is the cleavage of the signal sequence by the signal peptidase. The cleavage of the polypeptide weighs less then when the protein was attached to the signal sequence and before it grows in the ER lumen. This is evident from the diagram‚ since lighter proteins move father down the gel than do heavier ones. Given the length
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B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Protein Article Research John Doe SCI 241 May 17‚ 2012 Jane Doe Protein Article Research This class has been very informative during the past few weeks. We have learned how to count calories‚ read nutrition labels‚ use a tracker to monitor our daily intake‚ carbohydrates‚ and learned about proteins and their effects. According to the readings in the text and the article proteins are the building blocks in the body. I learned that protein is found in all tissues‚ cells and organs of the body
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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“Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is
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Task 1 • Describe the structure of an enzyme as a protein‚ in terms of tertiary/ quaternary structures. 1) Primary Structure This is in reference to the order of way that amino acids are connected to form a protein. These are built up from 20 amino acids‚ and follow these structures o A carbon (the alpha carbon) bonded to the four groups below: o A hydrogen atom (H) o A Carboxyl group (-COOH) o An Amino group (-NH2) o A "variable" group or "R" group 2) Secondary Structure This is in reference
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BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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April 22‚ 2015 Does protein help to build muscle? The latest craze is about protein and how it affects building muscles and building muscle strength. Today‚ there are many athletes that use it as a supplement. There are many protein supplements available that are claiming to help people who work out to build muscle faster. Protein is a structure within our bodies that consists of amino acids. The cells in our bodies need this to function properly. If we did not have protein‚ our body’s functions
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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