TITLE: The Amount of Protein in Chicken Tissues over Cooked Various Periods of Time. ABSTRACT: In this lab‚ we are using a BioRad protein assay dye to determine the concentration of protein in our chicken. The dye binds to the amino acid residues‚ which allow us to find the concentration of protein (BioRad Protein Assay for Tissues). Our hypothesis was the longer chicken is cooked the less protein is available. To test our hypothesis‚ we made samples using our chicken and distilled water to determine
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LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the stimulant for
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In this report‚ the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol
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Protein Article Research SCI/241 August 1‚ 2013 Dr. Theodore Keneklis Protein Article Research Proteins are made up of amino acids‚ and in our bodies we have proteins that “are part of every cell‚ tissue‚ and organ in our bodies” (Centers for Disease Control and Prevention: Protein‚ http://www.cdc.gov/nutrition/everyone/basics/protein.html). There are complete proteins which are made up from animals. These kinds of proteins are found in meat‚ poultry‚ fish and even cheese. A complete
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4A.2 RRL 4A.2.1 Coagulation of Proteins Coagulation of protein refers to sticking together‚ like a blood clot‚ usually as a result of denaturation or coming out of solution due to abnormal ionic strength or a change of solvent. Definite characteristics of the proteins are changed when they are coagulated‚ among which is loss of solubility in water and dilute salt solutions. In some instances and under certain conditions the coagulation process may be reversible. (Campbell‚ et.al‚ 1979) 4A.2.2
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in the fragmentation of the DNA. Protein glycosylation may be an additional damaging factor . In the attempt to repair DNA‚ poly(ADP-ribose) polymerase (PARP) is overstimulated. This diminishes cellular NAD+‚ and subsequently ATP‚ stores the depletion of the cellular energy stores ultimately results in beta cell necrosis. Although streptozotocin also methylates proteins ‚DNA methylation is ultimately responsible for beta cell death‚ but it is likely that protein methylation contributes to the functional
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Receptor Protein Protein that binds to a specific single molecule‚ enabling the cell to respond to the signal molecule. i.e. The muscles of a person exercising can not contract without receptor proteins and signal molecules that tell the muscles when to contract and when to relax. Second Messenger Signal molecule produced in response to the binding of a chemical signal. Acts as a signal molecule in the cytoplasm. Signal Molecule Carries information throughout the body and to other
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Sarah Khan Bivins-5th April 20th‚ 2013 Protein Synthesis Every day‚ you take in an abundance of different biomolecules; one of them being proteins. Have you ever wondered how proteins are made? They don’t just grow from a tree or fall from the sky‚ they are made through a process called protein synthesis. Protein synthesis is broken up in two two steps: transcription and translation. Transcription starts inside the nucleus when the DNA is unzipped by helicase. Following that‚ the mRNA nucleotides
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DENATURATION OF PROTEINS Abstract The experiment was done to be able to understand how various denaturants such as HCl and NaOH affects proteins. It was observed that different denaturants act upon or denature protein differently. This was determined using the principle of viscometry. An Ostwald viscometer was used to measure the viscosity of the prepared native‚ blank‚ denatured native and blank with denaturant solutions. The time required for the said solutions to pass through the viscometer
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Why are proteins important? Proteins can easily be addressed as one of the most important molecules in animal cells. There are many varieties of protein molecules which allow a vast range of biological activities in the cell.( Williams‚ G.(2000))’Proteins are big compound with large molecular masses’’(Williams‚ G.(2000)) whose main chemical constituents are hydrogen‚ carbon ‚oxygen and nitrogen. A protein structure consists of a specific sequence of amino acids called the
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