B I O L O G Y 130 INTRODUCTORY CELL BIOLOGY LECTURE NOTES Department of Biology University of Waterloo Fall‚ 2012 BIOL 130 LECTURE NOTES Fall‚ 2012 a Lecture Notes This booklet contains the notes that will be presented as part of the online modules. For copyright reasons‚ the figures that will be shown along with the notes cannot be reproduced. However‚ most of these figures come from the required course text‚ Cell and Molecular Biology: Concepts and Experiments‚ 6th edition
Premium Covalent bond Protein DNA
On researching new developments in the treatment of cystic fibrosis‚ I came across a very interesting article: “Ribosomal Stalk Protein Silencing Partially Corrects the ΔF508-CFTR Functional Expression Defect.” In the article‚ Viet et al1 reveal the potential application of the silencing of RPL12 (Ribosomal Protein L12)‚ to correct the ΔF508-CFTR biogenesis defect. As you know‚ cystic fibrosis is caused by a defect in the CF transmembrane conductance regulator (CFTR gene)‚ or more specifically‚ mutations
Premium DNA Gene Genetics
membrane structure. Inside the bilayer hydrophobic portions of proteins and phospholipids because the inside of the bilayer is nonaqueous. the proteins bob in the fluid bilayer of phospholipids. The proteins also vary in structure and function. There are teh integral proteins; transmembrane proteins with hydrophobic regions that cover the hydrophobic interior of the plasma membrane. There is also the peripheral proteins‚ they are not embedded in the membrane‚ but they are attached
Premium Cell membrane Lipid bilayer Protein
translucent grease mark on a brown paper bag. The objective for the Dye test was to test for lipids. When mixed with water and the tested substance‚ a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch‚ a reducing sugar‚ and effect of amylase on a cracker that
Premium Protein Glucose Fructose
A macromolecule can best be described as an immense molecule‚ made up of thousands of covalently bonded carbon atoms. There are four classifications of macromolecules: carbohydrates‚ lipids‚ proteins‚ and nucleic acids. Each classification performs specific functions that allow cells to grow and survive. Macromolecules can be either split or bonded through dehydration synthesis or hydrolysis. Dehydration synthesis occurs when water is released‚ electrons become shared‚ covalently bonding two
Premium DNA Protein RNA
Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples
Premium Glycerol Glucose Liver
hydrophobic tails having any contact with the aqueous solution. Membranes contain phospholipids‚ proteins and carbohydrates‚ which are arranged in a fluid mosaic structure. The proteins within the membrane are responsible for most of the membranes’ properties. For example‚ on the inside surface of cell membranes‚ proteins are attached‚ involved in maintaining the cell’s shape. These could be enzymes too. Proteins on the outside surface of cell membranes can act as receptors‚ such as antigens or hormones
Premium Cell membrane Protein
failure. 1. What makes transcription important? 2. What is the purpose of proteins? 3. How would a substance that affects transcription make the liver to stop functioning? 4. Why did it take Caesar two days to die? Claudius Caesar’s Death 1. What makes transcription important? mRNA transfers the code for proteins from DNA in the nucleus to ribosomes in the cytoplasm 2. What is the purpose of proteins? Proteins build cells‚ cause change in other cells‚ make enzyme to control functions
Premium Protein Claudius Enzyme
the chromosomes of organisms other than bacteria (i.e.‚ eukaryotes) are composed. It consists of protein‚ RNA‚ and DNA. Chromosome → a threadlike structure of nucleic acids and protein found in the nucleus of most living cells‚ carrying genetic information in the form of genes. Cristae → The infoldings or inward projections of the inner membrane of the mitochondrion‚ which are studded with proteins and increase the surface area for chemical reactions to occur like cellular respiration. Cytoplasm
Premium Cell Cell nucleus Eukaryote
Analysis: Lab 1 Identifying Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test‚ this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test‚ the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far
Premium Starch Carbohydrate Glucose