Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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strong inhibition of plant pathogens‚ Rhizoctonia sp. Botryosphaeria rhodina and two strains of Alternaria sp. (Alternaria sp. (A1) and Alternaria sp. (A2) - tomato wilt fungi) which was mainly due to presence of fungitoxic phenolic compound eugenol. When this eugenol was further subjected to agar diffusion assay it shows strong efficacy against Alternaria sp. (A1) and Penicillium chrysogenum with MIC value 0.16 and 0.31 mg/disc‚ respectively (Faria et al.‚ 2006). The plant fraction and essential oil
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CHAPTER 4 RESULTS AND DISCUSSION 4.1 Percentage yield Fresh leaves of C. nardus with 1 kg portion yielded about 22.57g of aqueous crude extract‚ which was approximately about 2.26% of yield. The result showed that fresh leaves had a higher percentage yield compared to the air-dried leaves‚ it was only 1.3% yield which conducted by Koba et al. (2009) with the similar extraction technique. There were other factors that might affect the percentage yield‚ such as influence of the harvest place
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REACTIONS OF HYDROCARBONS Vanessa P. Manibpel De La Salle University - Dasmariñas ABSTRACT Five substances namely Hexane‚ Eugenol‚ Unknown hydrocarbon 1‚ Unknown hydrocarbon 2‚ and Acetylene gas was used for the selective reactivity of hydrocarbons to functional group tests; Bayer’s test‚ Bromine test light‚ Bromine test dark‚ and Tollen’s test. In Bayer’s test‚ only Acetylene‚ Eugenol and Unknown hydrocarbon 2 reacted positively and the rest retain the purplish color of KMnO4. On the other hand‚ the
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and unspoiled. Antimicrobial properties of essential oils (EOs) reveal that Gram-positive bacteria are more vulnerable than Gram-negative bacteria. A number of EO components have been identified as effective antibacterials‚ e.g. carvacrol‚ thymol‚ eugenol‚ cinnamaldehyde and cinnamic acid‚ having minimum inhibitory concentrations (MICs) at higher dilutions in vitro. EOs comprise a large number of components and it is likely that their mode of action involves several targets in the bacterial cell. The
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are present between 3000 and 1700. The NMR when manipulated with a multiple of three‚ showed the presence of 9 hydrogens at 1.2 ppm and 3 hydrogens at 2.1 ppm. The typical NMR for pinacolone shows peaks around 20‚ 40‚ and 70 ppm due to the electronegativity of the oxygen present in the compound. The NMR of the organic product is inconsistent with the typical NMR of pinacolone or water. This could potentially be due to a mixture of the two compounds when the NMR was run and is due to the experimental
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and Safety Notices The Elements 1. Nitration of acetanilide 2. Hydrolysis of p – nitroacetanilide and thin layer chromatography 3. Separation of a three – component mixture by extraction 4. Completion of experiments from day 1 to 4 5. NMR workshop 6. Sandmeyer Reaction: Preparation of p-chloronitrobenzene 7. Synthesis of benzilic acid from benzil 8. Aldol Condensation: Preparation of ethyl cinnamate 9. Isolation of caffeine from tea 10. Completion of isolation of
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Experiment A Spectroscopic determination of Iron with Phenanthroline Unknown # 2 Mass of Mohr’s Salt: 0.2040 1. 5mL of 6M acetic acid was measured and transferred into a 100mL volumetric flask with a pipette and diluted to the mark. Concentration = [0.2040(±0.0001)g/100(±0.08)mL]*[(1mol/392.16g)/(1L/1000mL)] = 0.005202(±0.09382%) = 0.005202(±0.000005)mol/L 2. 10mL of the above stock solution was transferred to a 250 mL volumetric flask and diluted to the mark. Concentration
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benzene ring. The peaks occurring 1650 cm-1 showed the constituent contained at least one C=C bond between Csp2 molecules (Figure 1). After elimination the last choice of the compounds using the IR‚ the major constituent of clove oil was identified as eugenol (Diagram
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Department College of Science‚ De La Salle University-Dasmarinas ABSTRACT Five substances which are Hexane‚ Eugenol‚ Unknown hydrocarbon 1‚ Unknown hydrocarbon 2‚ and Acetylene gas was used for the selective reactivity of hydrocarbons to functional group tests; Bayer’s test‚ Bromine test light‚ Bromine test dark‚ and Tollen’s test. In Bayer’s test‚ only Acetylene‚ Eugenol and Unknown hydrocarbon 2 reacted positively and the rest retain the purplish color of KMnO4. On the other hand‚ the
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