Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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References: et al.‚ 2012). Our in vitro study evaluated the susceptibility of Pseudomonas aeruginosa Orthopaedics‚ 2011. 35(9): p. 1415-1420.) n 176B
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It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa 3. Slides 4. Inoculating loop 5. Dye solution a. Crystal violet b. Methylene blue c. Carbol fuchsin 6. Test tubes Procedure: 1. By using inoculating loop a minute of bacterial was transferred onto the glass slide. 2. Then a film
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cystic fibrosis prone to infections with Pseudomonas aeruginosa? Pseudomonas aeruginosa is an opportunistic infection that usually does not affect healthy patients‚ but is particularly detrimental in patients with cystic fibrosis. This is because they are antibiotic resistant to a certain extent. Paired with this‚ patients with cystic fibrosis have a decreased ability to produce mucous membranes‚ making it easier for biofilms associated with P. aeruginosa (Lyczak et al 2002). This along with their
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Agnihotri‚ N.‚ Gupta‚ V. and Joshi‚ R.M. (2004). Aerobic bacterial isolates from burn wound infections and their antibiograms: A five–year study Armour‚ A.D.‚ Shankowsky‚ H.A. and Swanson‚ T. (2007). The impact of nosocomially–acquired resistant Pseudomonas aeruginosa infection in a burn Landi‚ F.‚ Onder‚ G. and Russo‚ A. (2007). Pressure ulcer and mortality in frail elderly people living in community Landi‚ F.‚ Sgadari‚ A. and Bernabei‚ R. (1996). Pressure ulcers. Ann. Intern. Med.‚ 125(5): 422–425. Perencevich
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Investigating the Growth of P. fluorescens Biofilms at the Air-Liquid Interface and the Variations in Biofilm Integrity Abstract Pseudomonas fluorescens produces biofilms in a variety of environments‚ and in experimental situations has been shown to develop random mutations resulting in a phonotypical change in the resulting biofilm. One particular mutation resulted in the growth of a biofilm known as the Wrinkly Spreader‚ which produced a thicker‚ stronger biofilm that had a stronger attachment
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seeds and determines its inhibitory effect to the growth of Staphylococcus aureus‚ Staphylococci‚ and Pseudomonas aeruginosa. The Bitoon seeds were extracted for the purpose of Phytochemical analysis. Bitoon seeds were collected and shredded. Six treatments were prepared with three replicates each and applied to cultured bacteria which are Staphylococcus aureus‚ Staphylococci‚ and Pseudomonas aeruginosa‚. The treatments include 100%‚ 75%‚ 50%‚ 25% and the controlled substances Chloramphenicol for Positive
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Pseudomonas aeruginosa and Nosocomial Pneumonia Pseudomonas aeruginosa is the most common pathogen in nosocomial pneumonia. It is hospital acquired infection that the hospitals eat up the cost of treatment. Although pneumonia can be prevented‚ it is still an infection that we find in hospitals all across the United States. Pneumonia is a dangerous infection in patients who are already immunosuppressed and get pneumonia as a secondary infection. Preventative measures need to be taken to prevent
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Characteristics Microorganism 1. What is the causative organism (genus and species) for the disease? Legionella pheumophilia Family Pseudomonacea: Pseudomona aeruginosa Family Pasteurellaceae Haemophilus influenza Bordetella pertussis 2. Is the causative organism a virus‚ bacterium‚ protest‚ mold or yeast? Bacteria Bacteria Bacteria Bacteria 3. General characteristics. Morphology: Bacilli pleomorphic-( cocci ->filaments) varies Motility: flagella Gram
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