Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species are present‚ a blue-green or
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and applications of biosurfactant produced by Pseudomonas cepacia using de-oiled rice-bran Garima Bhardwaj1‚ Swaranjit Singh Cameotra2‚ Harish Kumar Chopra1* 1*Department of Chemistry‚ Sant Longowal Institute of Engineering and Technology‚ Longowal - 148106‚ Distt. Sangrur (Pb.)‚ India. E-mail: hk67@rediffmail.com Fax: 91-1672-280072; Tel: 91-1672-305204 (O) 2Institute of Microbial Technology‚ Sector-39-A‚ Chandigarh-160036‚ India Abstract: Pseudomonas cepacia MTCC 7099 was studied for the biosurfactant
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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Investigating the Growth of P. fluorescens Biofilms at the Air-Liquid Interface and the Variations in Biofilm Integrity Abstract Pseudomonas fluorescens produces biofilms in a variety of environments‚ and in experimental situations has been shown to develop random mutations resulting in a phonotypical change in the resulting biofilm. One particular mutation resulted in the growth of a biofilm known as the Wrinkly Spreader‚ which produced a thicker‚ stronger biofilm that had a stronger attachment
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Staphylococcus aureus and Pseudomonas fluorescens and determine their Gram classifications. Materials Broth cultures of Staphylococcus aureus and Pseudomonas fluorescenes‚ a Bunsen burner‚ one inoculating loop‚ one clean glass slide‚ one slide dish‚ bibulous paper‚ Gram-staining materials (Crystal Violet‚ Iodine‚ Ethyl Alcohol‚ Safranin‚ DI water)‚ and a microscope. Methods Smear Preparation Obtained a clean dry glass slide‚ Staphylococcus aureus and Pseudomonas cultures. Added drop
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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