Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
Premium Bacteria Microbiology Agar plate
Aseptic Technique is based on surgical conscience; that is‚ the ethical and professional motivation that regulates a professional’s behaviors regarding disease transmission. (Fuller) All patients are bound to get an infection. Certain situations can increase vulnerability‚ like disturbance of the body’s defenses like contradictions to anesthesia‚ severe burns or an immune disorder. A key difference between the operating room and other clinical environments is that the operating area has high
Premium Surgery Physician Medicine
Counseling Techniques Sarah B. Fluhart Bellevue University Abstract Counseling Techniques come in a wide variety of models and from many different theories of counseling. The counseling techniques described during any motivational interviewing process are the basic techniques that will be addressed in this paper. Motivational interviewing techniques include: Asking open-ended questions‚ using affirmations‚ forming reflective statements‚ and providing a summary‚ these techniques along with body
Premium Active listening Sentence Emotion
Brainstorming is a group creativity technique by which a group tries to find a solution for a specific problem by gathering a list of ideas spontaneously contributed by its members. The term was popularized by Alex Faickney Osborn in the 1953 book Applied Imagination. In the book‚ Osborn not only proposed the brainstorming method but also established effective rules for hosting brainstorming sessions. Brainstorming has become a popular group technique and has aroused attention in academia. Multiple
Premium
First‚ models of the object are generated. Next a viewing specification and lighting conditions are selected. Those surfaces visible to the viewer are then determined and rendered. Thick and thin line It is a popular technique that helps to make the drawing stand out. For this technique‚ thin lines are used in between adjoining surfaces. Thick lines are used when only one side can be seen. It is used to make the drawing stand out. Shading Shading is the process to make a simple line drawing of
Premium Analytic geometry Pen Drawing
Separation techniques are an important part of chemistry. However‚ their importance is not just limited to chemistry; they are also used in our daily lives. Separation techniques are methods used to separate and/or purify mixtures. There are many kinds of separation techniques that are used in our day to day life‚ such as filtration‚ Centrifuging‚ Decanting‚ fractional distillation and sieving. Each of these methods has their own specific uses in our daily lives which make our chores much easier
Premium Petroleum Distillation Water
Sean Tinsley Eng 151C-33 3/08/2013 Southern Gothic Fiction is a genre of literature unique to the American south. Major influences of the genre itself were the culture‚ religion‚ and economic standing of the south at the beginning of the 20th century. Many who read southern gothic are transported into a grotesque fantasy world where ideas of death‚ good versus bad‚ and god are all prevalent. Many American authors were greatly influenced by the ideas of southern gothic literature such as “Harper
Premium Southern Gothic Gothic fiction William Faulkner
job in relaying both messages to each party in need of an interpreter. One way to ensure this is expansion. There are several expansion techniques used by an interpreter to convey the meaning of the hearing person such as; contrasting‚ faceting‚ reiteration‚ explaining by example‚ using 3-D space‚ scaffolding and describe‚ then do. Contrasting technique is used by comparing two things‚ often one that is true and one that is false or visa versa. Research done on “High Context (ASL) v.s. Low
Premium American Sign Language Sign language Linguistics
Observation Techniques Observation techniques allow a researcher to observe behaviors and reactions in order to obtain information. The types of observation are direct versus indirect‚ disguised versus undisguised‚ structured versus unstructured‚ and human versus mechanical. Direct observation gives the opportunity for a researcher to observe behavior as it occurs and indirect observes the effects or results rather than the behavior itself. Disguised observation is when the subject is unaware
Premium Observation Psychology Philosophy of science
The Evolution of Science Fiction Worlds: From Frankenstein to Babel-17 One of the earliest successful science fiction stories was Frankenstein‚ written by Mary Shelley. Since the publishing of that novel‚ the world and imagery in science fiction novels that would follow would grow to encompass more expansive ideas‚ images‚ including other worlds‚ other dimensions‚ and vivid alien-like creatures to help tell these richly bizarre‚ yet human stories. In this paper‚ Mary Shelley’s Frankenstein‚ H.G
Premium Science fiction