The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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and Absolute Zero Determination Lab Report Experiment 4 Introduction In this experiment we measured the relationship between temperature‚ volume‚ and pressure. Objectives: Conduct a set of experiments‚ each of which illustrates a gas law. Gather data to identify the gas law described by each activity. Complete the calculations necessary to evaluate the gas law in each activity. From your results‚ derive a single mathematical relationship that relates pressure‚ volume‚ and temperature. Hypothesis:
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Introduction This report will investigate the six essential nutrients required in our diet. The report will provide the function of each nutrient and explore why they are vital for the body. Carbohydrates Carbohydrates are the most important nutrient for the body as it is the largest possible source of energy. Carbohydrate supplies the body with energy‚ which allows the brain to function more effectively and allows organs to operate sufficiently. When the body has energy this allows each
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An Interpretation of “On The Pulse Of Morning” The poem that Maya Angelou chose the read at President Clinton’s inaugural in 1993‚ delivers a message of hope. It uses the metaphor of morning or dawn to show that people can create their dreams and bring them to life. Maya Angelou has been a national celebrity since the reading of this poem. Her writings have a way of embracing people and successfully state her thoughts and emotions. “Pulse” is a call for hope and opportunity in part of our history
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A. Goal and Purpose: Session 1: In this lab‚ we will achieve a simple Friedel-Crafts alkylation of anthracene. The choice of anthracene as an aromatic substrate stems from two considerations. First‚ there is a question of regioselectivity. Second‚ anthracene and its derivatives are highly visible under UV light. Session 2: In this lab‚ we will complete a partial conversion of 9-acetylanthracene using m-chloroperoxybenzoic acid (mCPBA). We will also determine by NMR‚ the regiochemistry of the
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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Labs and Diagnostics Colonoscopy This test allows direct visualization of the small bowel‚ rectum‚ and colon. It is recommended for patients who have experienced fecal occult blood or obvious blood in the stool. It is also recommended for patients who have experienced a change in bowel habits or are experiencing abdominal pain. In patients who have had colorectal cancer‚ polyposis‚ or inflammatory bowel disease it is used to observe for continued progression of their condition. During the procedure
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Impurity Lab Report Aim: To find out the difference of the boiling point and melting point when adding impurity (salt) to water. Diagram: Method: 1.Set up the apparatus as shown in the diagram 2. Get a known amount of ice in the beaker (half full)‚ and a known amount of salt in another beaker 3. Measure the temperature (melting point) of the ice without adding any impurity (salt). 4. Measure the temperature (melting point) of the ice after adding the salt into the beaker. 5. Heat up
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December 8‚ 2013 Soil Analysis Lab Report Intro: Soil is produced over hundreds of years through the weathering of rocks and the decomposition of organic material. Soil contains nutrients that are taken from decomposed animals and plants which turn to detritus. Nutrients such as nitrogen are added to the soil in these ways by organisms such as nitrogen fixing bacteria. Soil is important because it is the fuel to the plants that we need in order to survive and for all species to survive. Soil
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lungs are a network of connected tubes that bring oxygen from the air into our blood‚ nourishing the trillions of cells that make up our bodies. The lungs also clean the blood of carbon dioxide waste created when cells use oxygen (Natural Geographic‚ 2012). In experiment one I hypothesize that the effect of respiration changes on carbon dioxide‚ concentration in exhaled air will be greatest the longer the respiration rate is increased. I predict that during normal breathing the time it takes to detect
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