devising a separation and purification scheme for a three component mixture. The overall objective is to isolate in pure form two of the three compounds. This was done using extraction‚ solubility‚ crystallization and vacuum filtration. The experiment was carried out two times‚ both of which were successful. Background Information: This experiment combined all the knowledge of the previous labs performed throughout the semester. An unknown mixture containing an organic acid or base and an organic
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Introduction Protein purification is the series of processes to isolate a single type of protein from a complex mixture. This is vital to extract and characterize the protein of interest. However‚ before doing so‚ it is important to release the protein from the subcellular organelles. This step is also known as homogenization. This step can be done with the use of blender. As the solution was homogenized‚ it may undergo saltation or acidation to remove impurities such as calcium anions. Hexane
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05/26/10 Elizabeth Angermyer Period: 4 Strong Acid and Weak Base Lab PURPOSE: To find the molarity of an unknown acidic acid. MATERIALS: 1. 250 mL beaker 2. 10 mL graduated cylinder 3. 50 mL beaker 4. Funnel 5. 125 mL Erlenmeyer flask 6. 50 mL buret 7. Ring stand 8. Strong acid 9. Weak base 10. Phenolphthalein 11
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synthesis of 4-hydroxy-4‚4-diphenylbutn-2-one was a three week multi-step project. The steps involved protecting the ketone of ethyl acetoacetate with a ketal group‚ followed by preparation and reaction with Grignard‚ then purification by extraction and recrystallization. The yield was 78.5%‚ but there are impurities present suggested by 1H-NMR‚ IR‚ and TLC. However‚ the results do indicate the desired hydroxyketone was formed. Introduction: The purpose of this multi-step lab was to synthesize 4-hydroxy-4
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Lab 4 Part 1: 10 points Assignment: Create an Internet and E-mail Acceptable Use Policy Learning Objectives and Outcomes Research and compare Internet and e-mail use policies enforced by other organizations. Create an Internet OR e-mail use policy for an organization. Assignment Requirements You are a networking intern at Richman Investments. An employee of the company used employer-owned equipment to access the Internet and check his personal Web-based e-mail account. He followed a
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Carboxylic acids are compounds which contain a carboxyl group‚ -COOH group‚ where the -COOH group is attached either to a hydrogen atom or to an alkyl group. One of the example of carboxylic acid is benzoic acid( benzene carboxylic acid) which has a benzene connected to the carboxyl carboxyl group‚ -COOH group. It has a formula of C6H5COOH. The carbon atom of a carboxyl group has a high oxidation state. Therefore‚ that many of the chemical reactions used for their preparation are oxidations. In
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hours. In the petri dish labeled tap water‚ 10 out of 10 seeds sprouted into plant growth as seen in Figure 5.1. The radish plant growth within the tap water petri dish grew so well it started to lift the lid off the dish. The results from the Acid Rain (50% vinegar solution) did not allow any radish seeds to germinate. There were never signs of seed germination while observing the seeds over a three week period. In the petri dish labeled Acid Rain‚ 0 out of 10 seeds sprouted into plant growth as seen
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1. Iron undergoes an interesting journey as it travels through the human digestive tract. Discuss the processes that occur in the stomach (pH 2) and the intestines (pH 8) and the pH dependence of each process. Foods that we consume contain Iron in the ferric (Fe3+ ) form. Ferric form is not usually absorbed in the body. In the stomach‚ there is low pH of 2 due to presence of gastric acids such as Hydrochloric and Ascorbic acids. These acids provide an acidic environment for reduction of ferric iron
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to study the effect acid rain has on seed germination and after performing this experiment is can be concluded that acid rain has a negative effect on seed germination. When seeds are soaked in an acidic solution the process of germination slows. Acidic solutions‚ while do slow growth of a seed‚ they do not kill the seed. A seed can be dormant for a majority of its lifespan‚ seeds watered with acid solutions stayed in a dormant condition. For example‚ trial three (3) part one (1) and two (2) both experienced
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Assessment Worksheet 65 Lab #4 – Assessment Worksheet Configure Group Policy Objects and Microsoft® Baseline Security Analyzer (MBSA) Course Name and Number: Student Name: Dennis Dobbins Instructor Name: P Butu Lab Due Date: 10/8/13 Overview In this lab‚ you used group policy objects to create a minimum password length password policy and link it to the newly created domain from the previous lab. You also ran the Microsoft® Baseline Security Analyzer (MBSA) and reviewed
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