smaller molecules will take a more convoluted path through the column and elute last. In this experiment‚ protein of interest which is LDH will be separated by using this gel filtration chromatography method. AIMS: 1. To do the final column purification of LDH using gel filtration chromatography with Sephacryl S-200 HR. 2. To determine the activity and the absorbance of LDH in gel filtration chromatography. 3. To determine the molecular weight of the LDH. MATERIALS AND METHODS Please refer
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Trials of other resins in the affinity column would determine which resin would have the greatest outcome in the purification process. The Ni2+-NTA-Agarose resin was effective for this experiment‚ but testing other resins can determine if the Ni2+-NTA-Agarose resin was the best resin to use in this purification process. It also needs to be determined if running the protein through the column would have any effect on the dialysis process. This information can be
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We underwent two different reaction schemes using Dipotassium phosphate (K2HPO4) and Potassium hydroxide (KOH) to isolate the compounds of pure Acetylsalicylic Acid/Aspirin and N-(4-hydroxyphenyl) Ethanimidate/Acetaminophen respectively from Excedrin. The first reaction scheme to undergo acid-base organic extraction was to isolate the most acidic compound in Excedrin with a weak base‚ K2HPO4. We isolated the aspirin using aqueous base extraction‚ with Dipotassium phosphate then treated with HCl
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Name: ___Kaici Holt_______ Stage Theorists of Human Development Worksheet Fill in this chart with the names of theorists and stages which occur in each theory‚ in each period of life. Notice that some boxes are merged such as Adolescence through Late Adulthood in the Psychosexual Theory because this time frame all encompasses only 1 of Psychosexual’s stages. The answers to this worksheet can be found in Chapter 1 of your textbook. EXPERIMENT WORKSHEET The following is an example of
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of water‚ I follow-up with the best organic coffee. For now‚ it has replaced my morning cup of green tea. Studies on caffeine continue and as each one comes to light‚ drinking coffee is no longer the dreaded bearer of health problems. For years‚ I didn’t drink coffee I avoided the caffeine. Because I like to keep my body clean and mean and I was under the impression the caffeine leached calcium from bones. However‚ I now know this is not the case. If you are healthy‚ one or two cups of coffee a
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Today’s world is moving faster and faster. Not too often will you find someone drinking coffee to stay awake. Companies today have created a drink that will kick the effect of being tired and feeling drowsy. Energy drinks today come in different sizes and names. They are becoming a growing problem in America today. Coffee is normally considered to be your more older generation not something really suited for a younger crowed like teenagers. But Energy drinks like Monster ‚Red Bull‚ AMP and Rockstar
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world today‚ there are many chemicals that people use to fix or help with issues within their own bodies. Some of these chemicals may include things like caffeine‚ nicotine‚ and alcohol. These supplements can have substantial effects on other things within the body like the brain‚ liver‚ and especially the heart. For example‚ many chemicals like caffeine‚ nicotine‚ aspirin‚ and sleep aid all have effects on the body which can cause your heart rate to increase outside of normal range. Other supplements
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the market the most popular are Red Bull and Monster. People are now mixing these with alcohol which has a serious side effect. There are several ingredients in energy drinks: caffeine‚ taurine‚ b vitamins‚ inositol‚ ginseng‚ glucuronolactone‚ artificial sweeteners‚ ginkgo biloba‚ and l-carnitine. The caffeine in these drinks can cause dizziness‚ jitters‚ nausea‚ irritability and nervousness. You can also have an allergic reaction like: a rash‚ difficulty breathing‚ and swelling of the mouth
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due to its bad impact on human health also not considered as a good approach to get instant energy. It may cause sudden increase in heartbeat‚ seizures and insomnia‚ that’s why most of the countries or states ban energy drinks containing 180 mg of Caffeine (Watson‚ 2013‚ pp. 37-42; Kevin‚ 2013). The most current example of this is the ban of energy drinks in Chicago by its government (Judy‚ 2013). In this essay which is basically an investigative essay we are going to investigate energy drink’s bad
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A 16 oz. can energy drink may contain 13 teaspoons of sugar and the same amount of caffeine found in 4 or more colas. 2. The sudden energy rush you feel after drinking one of these cans is often followed by a “crash-and-burn” effect which could leave you craving more. 3. While these drinks give your metabolism a quick boost‚ afterwards your stomach is left with the carbohydrates and caffeine neither of which are metabolism boosters. E. Energy drinks help you with your
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