The Separation and Purification of Organic Compounds Sunshine Marie Introduction Each year‚ many thousands of new or unknown organic compounds are prepared or extracted from natural sources such as plants‚ fungi and animals. In order to identify an unknown organic compound or the components of a mixture of organic compounds‚ it is necessary firstly to separate and purify the compounds. At any given temperature‚ a certain number of molecules of a substance in the liquid state have sufficient
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DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification Maintain the PCR volume of 50µL-100 µL. Pipet the elution buffer in the center of the PureLink® Spin Column (PSC) and perform an incubation. Add B2 to PCR reaction. Add this sample to the PSC. Centrifuge the PSC. Replace the PSC into the wash
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Cost Allocation at Water Purification Group Mary May pushed the door to her office thinking about her plan to enrol her daughter for a medical degree at a private university. The private university is the only institution of higher learning that her daughter can apply for‚ considering her high school results. Mary sat down at her table and a smile came to her lips. Her financial situation will definitely improve after the company‚ Bio-Organics‚ announce this year’s bonuses. Project ORG7 she
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Title: Purification of Egg white protein Name: Michael Johnson Partner: David Logad & Nandita Date: 2nd 9th September 2004 Group: Thursday 11:30am - 3:30pm Introduction Salting Out In 1888 Hofmeister that it can be possible to dehydrate a protein by adding salt to the solution‚ salting out. When a protein in a aqueous solution it is surrounded by water‚ in fact there can be up to 0.35g of water tightly bound to 1g of protein (Simpson 2004). Also the effectiveness of the salting out
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Water Treatment Nanotechnology‚ the engineering and art of manipulating matter at the nanoscale (1-100 nm)‚ offers the potential of novel nanomaterials for the treatment of surface water‚ groundwater and wastewater contaminated by toxic metal ions‚ organic and inorganic solutes and microorganisms. Due to their unique activity toward recalcitrant contaminants many nanomaterials are under active research and development for use in the treatment of water. Nanotechnology water treatment
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Separation and Purification of Organic Compounds Codilla‚ Christine‚ Cristobal‚ Marian‚ Dionisio‚ Jermaine‚ Dumaquita‚ Vanessa‚ Edrosolo‚ Lyka‚ Esquivel‚ Christine1 1University of Centro Escolar‚ Makati Date Performed: December 5‚ 2012 Date Submitted: December 17‚ 2012 Abstract The distillation process was successfully done because of the separation of water from the acetone. When the distillate was lighted with the matchstick it produced flame which indicates that there was more presence
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2012 [12] Brooks‚ D. D. (1991). Some perspectives on deep- fat frying. INFORM‚ 2: 1091-1095. [13] Buck‚ D.F. (1981). Antioxidants in soy oil. Journal of the American Oil Chemical Society‚ 58‚ 275–278. [14] Buczek‚ B and Chwialkowski‚ W.(2008). Purification of the used palm oil by adsorption. Polish Journal of Chemical Technology‚ 10‚ 1‚ 19 — 21. [15] Carr‚ R.A. (1978). Refining and degumming systems for edible fats and oils. J. Am. Oil Chemists ’Soc. 55‚ 765-771. [16] Coultate‚ T. P.(2002). Food:
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The Purity and Purification of Solids Recrystallization ABSTRACT In this experiment‚ impure adipic acid is purified though recrystallization. The impure adipic acid is dissolved in a solvent. After cooling it is ran through a vacuum filter to separate any moisture. The dry crystals are then weight for percent yield and the melting point range is taken to test the purity. There were two different trials ran in this experiment. The difference between the two was the addition of charcoal in
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