Experiment 55: A Separation and Purification Scheme The final lab report/procedure write-up will consist of the following: Report guide: 1. List the names‚ structures‚ and amounts of each compound in your mixture. 2. Draw a flow chart (like pg 687 in PLKE) showing how you separated and isolated your two compounds. Be sure to include the structures of the compounds at each step. 3. Write a procedure. This should include exactly how you performed your separation. It should have enough
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test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination followed by (proline:hydroxypr condensation oline gives a yellow color) Yellow sol’n/ppt. Nitration via SEAr with conc. HNO3‚ orange with excess NaOH Millon’s rgt.(Hg2+ & Hg22+nitrates& nitriles/Hg(NO3)2 in conc. HNO3‚ w/ trace of HNO2/HgSO4‚H2SO4 ‚NaNO2) Hopkins-Cole Test Glyoxylic acid (Mg powder‚ oxalic
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The Purity and Purification of Solids Melting Point Lab Introduction: The point of this lab was to determine the eutectic point for the naphthalene biphenyl mixture‚ as well as determining the melting point of an unknown substance by comparing it with two known samples. Melting point is a temperature in which a substance changes from solid state to liquid state. Melting points are used to determine whether the given substance is pure or not. Substances that melt sharply‚ less than 1-2°C indicates
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Deloris Buliner RESL201 Christopher Myers October 20‚ 2014 Purification of Judaism and Hinduism Judaism and Hinduism have different cultures and originate from different ends of the spectrum of religious development. The origin of Judaism is characterized by a dramatic break with the near Eastern religious tradition comprising of the unity of God‚ man‚ and the cosmos while Hinduism on the other hand‚ characterized by “pantheism” as the main motif in its religious outlook (Ridgeon‚ Lloyd). While
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Exercise No. 8 CARBOXYLIC ACIDS AND ACID DERIVATIVES I. OBJECTIVES: • To investigate the physical and chemical properties of Carboxylic acid and its derivatives • To understand the reactions of carboxylic compounds and derivatives. II. EXPERIMENTAL RESULTS Solubility of Carboxylic acids in 10% NaHCO¬3 Acetic acid - formation of bubbles Benzoic acid - formation of bubbles Test for Acetic acid NaOH + Acetic acid - blue litmus paper turned red NaOH + Acetic acid + FeCl3 - red colored
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The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff
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liquid form upon heating. The melting point of benzoic acid‚ the aromatic carboxylic acid was 122°C. Thus‚ the product contained impurities which caused the solid to melt at a lower temperature than the actual temperature. Pure solids typically melt at higher temperatures while impure solids melt at lower temperatures. This is due to the fact that a solid cannot form when it is held together by tight intramolecular forces in its pure state. In its impure form‚ the intramolecular forces are weaker since
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The Purity and Purification of Solids Melting Points CH-337 Abstract: The purpose of this experiment was to find the eutectic point for the mixture of naphthalene and biphenyl. This was done by measuring the melting point of both pure substances and five mixtures of different ratios. The purpose of the second section was to identify an unknown sample by measuring its melting point and mixing it with two known substances and comparing them. The results suggest that the eutectic point is closest
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Acid Base Titration bre’ana March 1‚ 2013 Purpose: The purpose of this experiment is to determine the concentration of a solution of NaOH by titration with a standard solution of HCl. It was also the purpose to determine the concentration of a sample of white vinegar by titration with a standard solution of NaOH. Introduction: Using the method of titration is how the experiment gets to the conclusion of the concentration of a solution. When doing this experiment‚ measurement is very important
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from a mixture by recrystallization. Compare the melting points of crude and recrystallized acetanilide. Introduction : The most common method of purifying solid organic compounds is by recrystallization. In this technique‚ an impure solid compound is dissolved in a solvent and then allowed to slowly crystallize out as the solution cools. As the compound crystallizes from the solution‚ the molecules of the other compounds dissolved in solution are excluded from the growing crystal
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