Anhydride Figure 11.3. The balanced chemical reaction of the formation of aspirin. Because the reaction is slow in pure acetic anhydride‚ the catalyst‚ commonly strong acids like phosphoric acid was used for the reaction. According to Le Chatelier’s principle‚ the presence of excess acetic anhydride forces the equilibrium towards the desired product‚ which in this case is the aspirin. In addition to this‚ the catalysts were also used to ensure that side reactions‚ which may
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Synthesis of Methyl Salicylate from Aspirin Esters are a class of organic compounds. An ester compromises an organic or inorganic acid in which one or more hydroxy (OH) groups have been replaced by and alkoxy (O-alkyl) group. For example‚ the simplest ester‚ methyl formate (CHOOCH3)‚ is made up of formic acid (CHO-OH) in which the hydroxyl group has been replaced by a methoxy group (-OCH3). Similarly‚ ethyl acetate (CH3CO-OCH2CH3) is made up of acetic acid (CH3CO-OH) in which the hydroxy group
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Manual Results To find out the identification of the unknown‚ 3 separate trials had to be performed to the unknown‚ some standards and a mixture of 50/50 of the unknown and the standard‚ or in the case of trial 3‚ both standards of aspirin & acetylsalicylic acid were combined in a 50/50 mixture. (See Table 1 below). * = Standards. In each trial each separate component was placed in its own capillary tube‚ and then a 3rd capillary tube contained a 50/50 mixture of both ingredients
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Experiment: The OH group on the benzene ring in salicyclic acid reacts with acetic anhydride to form an ester functional group. Thus the formation of acetylsalicyclic acid (aspirin) is referred to as an esterification reaction‚ which requires the presence of H+ (H2SO4 in our case). The technique used to purify the aspirin content is called crystallization‚ which consists in allowing the reaction to cool‚ decreasing the solubility of acetylsalicyclic acid and allowing it to crystalize. To purify
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affinity chromatography which the nickel sticks to the His-tag‚ after that eluate imidazole‚ nxt we do size exclusion which separates small from big‚big comes off first the imidazole captures it in the column next we do spectrophotometry. Then SDS-PAGE electrophoresis‚ size purity‚enzyme test does its function. Finally we did DHFR enzymatic assay. In the SDS-PAGE electrophoresis we load the gel up with Laemmli buffer‚ precision plus protein standard‚desalted eluate page‚ eluate page‚ wash page‚ flowthrough
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Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method: 1. Firstly mark a line across
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was carried out to see how the hydroxyl group on the benzene ring in salicylic acid reacts with acetic anhydride to form an ester‚ and to make aspirin. Synthesis of Acetylsalicylic Acid occurs by protonation of carbonyl (C=O) group‚ and a nucleophilic attack of OH on the acetic anhydride. The ferric chloride test and melting point were used to test the purity of the results. A hypothesized recovery rater of above 50% was expected. salicylic acid acetic anhydride
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percentage yield: Once the percentage yield is calculated then the quality of the apparatus and procedure can be determined to obtain better results and more product in the future. The purpose of this experiment‚ is essentially to determine the purity of the product that is collected and filtered and recrystallized. Experimental Procedure: The experimental procedure used for this experiment was outlined in the CHEM 123L lab manual‚ Experiment #1. All steps were followed without deviation. Experimental
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which water is processed and treated. This experiment will involve removing chemical impurities in water through using techniques such as absorption spectrophotometry‚ sample collection‚ serial dilution‚ filtration PH and graphical analysis. If this experiment is done properly the water sample collected from the pond should wind up purified and have a purity percentage close to 100%. Procedure: The steps of this experiment can be found in the Lab Manual. Dillon‚ Stephanie. “The Purification of Water
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The Visible Spectra Of Orange Soda Pop Objective : To measure an absorbance value of the different colour solutions by using Spectrophotometry Introduction Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer (a device for measuring light intensity) that can measure intensity as a function of the color‚ or more specifically‚ the wavelength of light (nm). The instrument also can be use on biological sample such as chlorophyll pigments and
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