Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
Premium Molecular biology Gel electrophoresis DNA
Current Regulations for Standardization of Crude Drugs In India a great deal of bulk knowledge exists among ordinary people about the traditional use of herbal medicine. It is difficult to quantify the market size of the traditional Indian system. Since most practitioners formulate and dispense their own recipes. The present annual turnover of product manufactured by large companies is estimated at approximately US $ 300 million compared to a turnover of approximately US $ 2.5 billions for modern
Free Chromatography Thin layer chromatography Analytical chemistry
“More Than Skin”: A Literary Analysis of The Great Gatsby Miss Transue Block 1 April 17th‚ 2014 Magnificent clothes are sought after‚ fought over‚ and talked about. When a person dresses nicely‚ he or she is respected. It is possible that this respect could have been formed under false pretenses. Appearances can be fake and deceiving. Clothes can hide things other than skin. In the novel The Great Gatsby‚ F. Scott Fitzgerald uses images of impressive clothing to mask despicable
Premium The Great Gatsby F. Scott Fitzgerald Jay Gatsby
Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
Premium Molecular biology DNA Plasmid
concept of cleanliness differs from purity[disambiguation needed]‚ which is a physical‚ moral‚ or ritual state of freedom from pollutants. Whereas purity is usually a quality of an individual or substance‚ cleanliness has a social dimension‚ or implies a system of interactions.[2] "Cleanliness‚" observed Jacob Burckhardt‚ "is indispensable to our modern notion of social perfection."[3] A household or workplace may be said to exhibit cleanliness‚ but not ordinarily purity; cleanliness also would be a characteristic
Premium Cleanliness Immune system Hygiene
many areas of chemistry especially‚ in organic chemistry area because the melting point is really significant in order to identify the purity and the identity of a substance. Measuring the melting point is a fast and cost-effective technique which remains a strong link to the vast pre-instrumental chemistry literature‚ so the technique is still used for measuring purity of organic and pharmaceutical compounds. For that reason‚ determination of the melting point is more than just a classroom exercise
Premium Chemical compound Chemistry Melting point
Explore the presentation of Mina Most critics would safely agree that “Dracula” is a novel that dramatically presents the role of the female in Victorian society. Bram Stoker was writing this novel at the end of the nineteenth century when Victorian women were placed into three distinct categories‚ all three relating to their sexual behaviour. She was either a virgin‚ so pure and innocent; a mature wife or mother; or if she was neither of these she was considered a “loose woman” and so was neglected
Premium Dracula Abraham Van Helsing Count Dracula
is and where she is from. Whoever decides to defy the components that made up a true womanhood was shunned as an enemy of the god of civilization and the republic. The components and attributes that made up a true woman was defined by her piety‚ purity‚ submissiveness and domesticity. If all components were present‚ true womanhood would spell out mother‚ daughter‚ sister‚ wife and woman ; which all led to a promise of happiness and power. Piety was the main attribute of a woman’s virtue or the
Premium Woman Virtue Female
ultimate goal. But to use mathematics to produce solutions to a physical system at its purest level would require overwhelmingly complex maths‚ considering the number of variables in the system. This is why Physics would be considered the next level of purity. Applying basic trigonometrical mathematics to an architectural project is more than sufficient‚ and it’s very rare we need to introduce variables such as the building materials into these
Premium Epistemology Religion World
something sacred and worthy of praise. Being a virgin is a symbol of innocence‚ highly valued by religions and encouraged by them to remain so; it is something that only the bond of marriage is a worthy reason for its loss. Virginity is a symbol of purity‚ the intactness and immaculacy of the body and soul. It represents the body has remained untouched by sexual acts and the soul has remained untainted by sexual urges; both remain unmarked by acts of foreign mind and touch. Virginity is the prominent
Free Human sexuality Sexual intercourse John Milton